Polysaccharides as Quality Marker to Rapid Profile for by PXRD.

Background: has long been recognized as a mysterious and valuable traditional Chinese medicine but there has been little research on quality markers for .

Purpose: This study looked into the potential of using powder X-ray diffractometry (PXRD) to analyze polysaccharides as a quality marker for .

Study Design: There were 16 different habitats of collected in Qinghai, Gansu, Sichuan, Yunnan, and Tibet. In addition, five different types of species were collected. The characteristic diffraction peaks of were determined and then matched with the characteristic diffraction peaks of intracellular polysaccharides obtained from to determine the attribution relationship of the characteristic diffraction peaks.

Methods: powder's X-ray diffraction pattern is determined by its composition, microcrystalline crystal structure, intramolecular bonding mechanism, and molecular configuration. After fractionation and alcohol precipitation of crude intracellular polysaccharide, mycelium crude intracellular polysaccharide (MCP) and fruiting body crude intracellular polysaccharide (FCP) were obtained and the fingerprint of was identified by the specific characteristic peaks of the X-ray diffraction pattern from intracellular polysaccharide.

Results: The results indicated that the PXRD patterns of different populations of were overlaid well with 18 characteristic diffraction peaks obtained by microcrystalline diffraction. Moreover, the powder diffractograms as a fingerprint provided a practical identification of from other species. In addition, we detected that the powder diffractograms of intracellular polysaccharide MCP and MCP75 could be coupled with the PXRD of . Specifically, 18 characteristic diffraction peaks were identified as coming from MCP and MCP75 according to those interplanar crystal spacing, which matched well with those of PXRD of .

Conclusions: PXRD spectra combined with an updated multivariable discriminant model were found to be an efficient and sensitive method for quality control. According to the findings of this study, PXRD should be further investigated for quality control assessments and plant extract selection trials.