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Enzymatic glycosylation of aloesone performed by plant UDP-dependent glycosyltransferases. | LitMetric

Enzymatic glycosylation of aloesone performed by plant UDP-dependent glycosyltransferases.

Glycobiology

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Søltofts Plads 220, Lyngby DK-2800, Denmark.

Published: July 2024

AI Article Synopsis

Article Abstract

Aloesone is a bioactive natural product and biosynthetic precursor of rare glucosides found in rhubarb and some aloe plants including Aloe vera. This study aimed to investigate biocatalytic aloesone glycosylation and more than 400 uridine diphosphate-dependent glycosyltransferase (UGT) candidates, including multifunctional and promiscuous enzymes from a variety of plant species were assayed. As a result, 137 selective aloesone UGTs were discovered, including four from the natural producer rhubarb. Rhubarb UGT72B49 was further studied and its catalytic constants (kcat = 0.00092 ± 0.00003 s-1, KM = 30 ± 2.5 μM) as well as temperature and pH optima (50 °C and pH 7, respectively) were determined. We further aimed to find an efficient aloesone glycosylating enzyme with potential application for biocatalytic production of the glucoside. We discovered UGT71C1 from Arabidopsis thaliana as an efficient aloesone UGT showing a 167-fold higher catalytic efficiency compared to that of UGT72B49. Interestingly, sequence analysis of all the 137 newly identified aloesone UGTs showed that they belong to different phylogenetic groups, with the highest representation in groups B, D, E, F and L. Finally, our study indicates that aloesone C-glycosylation is highly specific and rare, since it was not possible to achieve in an efficient manner with any of the 422 UGTs assayed, including multifunctional GTs and 28 known C-UGTs.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273223PMC
http://dx.doi.org/10.1093/glycob/cwae050DOI Listing

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