AI Article Synopsis
- Insect cell lines, specifically Sf21 cells, are being explored for their potential in ecotoxicity testing, which hasn’t received much focus compared to other applications.
- The study demonstrated that Sf21 cells are sensitive to the fungicides Propiconazole and CuSO, with significant effects noted at specific concentrations, indicating their potential as an acute toxicity testing tool.
- The findings showed that a 0.5% concentration of the solvent DMSO did not negatively impact cell viability, suggesting that Sf21 cells can be effectively used in toxicological assessments.
Article Abstract
Insect cell lines are finding utility in many areas of biology, but their application as an in vitro tool for ecotoxicity testing has been given less attention. Our study aimed to demonstrate the utility and sensitivity of Sf21 cells to commonly used fungicides: Propiconazole and CuSO, as well as dimethyl sulphoxide (DMSO) an industrial solvent. Sf21 cells were readily cultured from frozen stocks in 3-4 days and showed utility as an invertebrate in vitro acute toxicity test. The data showed the threshold levels of cell survivability against propiconazole and CuSO. The EC values were 135.1 μM and 3.31 mM respectively. The LOAEL (lowest observed adverse effect level) was ≈ 1 μM for propiconazole and ≈ 10 μM for CuSO. Culturing of Sf21 cells in media containing the solvent DMSO showed that 0.5% DMSO concentration did not effect cell viability. Sf21 cells are sensitive and useful as a robust ecologically relevant screening tool for acute toxicity testing.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11399169 | PMC |
| http://dx.doi.org/10.1007/s10646-024-02781-9 | DOI Listing |
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