AI Article Synopsis
- Researchers studied ribosomal RNA (rRNA) processing in yeast using a technique called CircTA-seq, which allows for detailed analysis of rRNA intermediates at single-molecule and single-nucleotide levels.
- They discovered that the processing of 5.8S rRNA involves converting a long form into a short form and that initial trimming relies on proteins Rex1, Rex2, and Trf4.
- The study also highlighted the complexity of 25S rRNA processing involving multiple Rex proteins, and it revealed how polyadenylation of pre-rRNAs affects their degradation efficiency and subsequent re-polyadenylation by the exosome.
Article Abstract
Ribosomal RNAs are processed in a complex pathway. We profiled rRNA processing intermediates in yeast at single-molecule and single-nucleotide levels with circularization, targeted amplification and deep sequencing (CircTA-seq), gaining significant mechanistic insights into rRNA processing and surveillance. The long form of the 5' end of 5.8S rRNA is converted to the short form and represents an intermediate of a unified processing pathway. The initial 3' end processing of 5.8S rRNA involves trimming by Rex1 and Rex2 and Trf4-mediated polyadenylation. The 3' end of 25S rRNA is formed by sequential digestion by four Rex proteins. Intermediates with an extended A1 site are generated during 5' degradation of aberrant 18S rRNA precursors. We determined precise polyadenylation profiles for pre-rRNAs and show that the degradation efficiency of polyadenylated 20S pre-rRNA critically depends on poly(A) lengths and degradation intermediates released from the exosome are often extensively re-polyadenylated.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417381 | PMC |
| http://dx.doi.org/10.1093/nar/gkae606 | DOI Listing |
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