AI Article Synopsis
- Researchers developed a 38-colour high dimensional immunophenotyping panel to analyze various immune cell subsets in peripheral blood mononuclear cells (PBMCs).
- This panel allows for the identification of innate and adaptive immune cells, including different T and B cell types, as well as assessing their activation status.
- Testing on PBMCs from 15 healthy adults showed that this tool is critical for studying immune cells in health and disease, especially when sample availability is restricted.
Article Abstract
High dimensional immunophenotyping panels are invaluable resources for performing extensive phenotyping on peripheral blood mononuclear cells (PBMCs). We designed a 38-colour high dimensional phenotyping panel to measure innate (monocytes, dendritic cells, NK cells, basophils, innate like lymphoid cells), T cell (γδ T cells, MAIT cells, CD4 and CD8 memory, Th1, Th2, Th17, Tfh, Treg) and B cell (memory, plasma cells, transitional B cells, plasmablasts, IgG, IgM) subsets in addition to their activation status using the 5-laser Cytek Aurora. We optimised optimal fluorochrome combinations and titres to minimise spread and autofluorescence of rare immune cell populations and tested this panel on PBMCs from 15 healthy adults. This high dimensional panel will be invaluable for direct ex vivo studies to evaluate immune cells in the context of human health and disease, especially when samples are limited.
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| http://dx.doi.org/10.1016/j.jim.2024.113726 | DOI Listing |
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