The Flavonoid Glycoside from Hance Alleviates Alcoholic Liver Injury by Inhibiting Ferroptosis in an AMPK-Dependent Manner.

Hance is a vegetative food and can be used as a folk beverage or soup to clear liver toxins and prevent liver damage. However, the components and effects of Hance in alcohol-induced liver injury were unknown. This study aimed to obtain abundant phytochemicals from Hance and identify the potency of the isolates in preventing alcohol-induced liver injury. Alcohol-stimulated AML12 cells and Lieber-DeCarli diet-fed mice were used to establish and models, respectively. Our findings indicated that flavonoid glycosides, especially , could significantly alleviate alcohol-induced liver injury by inhibiting oxidative stress. Furthermore, we demonstrated that inhibited ferroptosis induced by lipid peroxidation. Mechanically, we found that regulated nuclear factor erythroid 2-related factor 2 (NRF2) expression via activation of AMP-activated protein kinase (AMPK) signaling. These results indicate that Hance is a great potential functional food for alleviating alcohol-induced liver injury.