AI Article Synopsis
- Healthcare facilities generate large amounts of waste, particularly from diagnostic plastics, prompting the need for more sustainable solutions.
- A new detection platform replaces traditional assay plates with a membrane in a portable device, enabling faster and greener detection of the p75 biomarker associated with ALS prognosis and severity.
- The new method allows for quick results in just 5 minutes while significantly reducing the amount of detection antibody used compared to conventional assays.
Article Abstract
Healthcare facilities produce millions of tons of waste annually, with a significant portion consisting of diagnostic plasticware. Here, we introduce a new detection platform that completely replaces traditional assay plates with a piece of membrane, offering a much greener and more sustainable alternative. The membrane, integrated within the portable vortex fluidic device (P-VFD), enables rapid detection of a clinically relevant protein biomarker, urinary p75. This biomarker is utilized to evaluate the prognosis, disease severity, and progression of amyotrophic lateral sclerosis (ALS). This assay has a limit-of-detection (LOD) of 4.03 pg, which is comparable to the plate-based assay (2.24 pg) and has been optimised through a full factorial design of experiments (DOE) and response surface methodology (RSM). P-VFD has great potential in quantifying p75 in human biofluids and can significantly reduce the assay time to 5 min compared to the current plate-based p75 ELISA assay (3 days), with at least a 4.4-fold reduction in the usage of the detection antibody.
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| http://dx.doi.org/10.1002/cssc.202401008 | DOI Listing |
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