AI Article Synopsis
- Extracting high-quality RNA from cotton is tough due to substances like polyphenolics and polysaccharides that interfere with the process.
- A new RNA extraction method using Triton-X-100 and Polyvinyl pyrrolidone (PVPP) has been developed, which effectively removes these problematic compounds and allows for successful RNA precipitation with Lithium chloride (LiCl).
- The study evaluates this new RNA extraction technique by synthesizing cDNA from cotton CMS lines and examining mitochondrial genes linked to male sterility, highlighting its advantages and limitations compared to existing protocols.
Article Abstract
The extraction of high-quality RNA from cotton (Gossypium spp.) is challenging because of the presence of high polyphenolics, polysaccharides, quinones, and other secondary metabolites. A high-throughput RNA extraction protocol is a prerequisite. This Triton-X-100-based RNA extraction method utilizes Polyvinyl pyrrolidone polymer (PVPP) treatment which efficiently removes phenolics, and the application of Lithium chloride (LiCl) has been found that successfully precipitated the high-quality RNA from cotton tissue. Cytoplasmic male sterility (CMS) is a maternally inherited trait associated with specific mitochondrial genome rearrangements or mutations. The suitability of RNA extracted from Cotton CMS lines was assessed. cDNA was synthesized from RNA and assayed for mitochondrial genes (cox3, nad3, nad9) associated with male sterility. This paper discuss the advantages and limitation of this protocol over existing protocol for RNA extraction for polyphenolics-rich plant tissue.
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| http://dx.doi.org/10.1016/j.ab.2024.115604 | DOI Listing |
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