AI Article Synopsis
- - The preparation of paraffin sections is critical in animal histological research, focusing on factors like dehydration time, waxing time, and drying temperature to ensure quality.
- - Optimal results were achieved with horse testis tissue using a 6-hour gradient dehydration, 60-minute wax immersion, and 15-minute drying at 75 degrees Celsius.
- - This study presents refined techniques that could streamline histological experiments specifically related to equine testes.
Article Abstract
The preparation of paraffin sections is an important experimental technique in animal histological research, and key factors that determine the quality of a section include the dehydration time, waxing time, and drying temperature of the paraffin section. Paraffin sections obtained from testis tissue of adult horses exhibited higher quality with clear tissue structure and complete cell morphology after they underwent gradient dehydration for 6 hours, were immersed in wax for 60 minutes, and were dried in a 75-degree oven for 15 minutes. The detailed, optimized procedures that are developed in the current study may simplify histological experiments and research on equine testes.
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| http://dx.doi.org/10.14670/HH-18-775 | DOI Listing |
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