AI Article Synopsis
- - Generated a comprehensive genetic assembly for 27 distinct
- Citrullus
- genotypes, expanding the previous reference genome by adding significant DNA and thousands of genes, showcasing enhanced genetic diversity.
- - Conducted comparative analysis revealing gene variants and structural changes, contributing to the understanding of watermelon evolution and traits like sweetness and bitterness, while also impacting disease resistance.
- - Successfully integrated disease-resistant genes from wild species (
- Citrullus amarus
- and
- Citrullus mucosospermus
- ) into cultivated watermelon (
- Citrullus lanatus
- ), providing new insights into watermelon genetics and potential for genetic improvement.
Article Abstract
To decipher the genetic diversity within the cucurbit genus Citrullus, we generated telomere-to-telomere (T2T) assemblies of 27 distinct genotypes, encompassing all seven Citrullus species. This T2T super-pangenome has expanded the previously published reference genome, T2T-G42, by adding 399.2 Mb and 11,225 genes. Comparative analysis has unveiled gene variants and structural variations (SVs), shedding light on watermelon evolution and domestication processes that enhanced attributes such as bitterness and sugar content while compromising disease resistance. Multidisease-resistant loci from Citrullus amarus and Citrullus mucosospermus were successfully introduced into cultivated Citrullus lanatus. The SVs identified in C. lanatus have not only been inherited from cordophanus but also from C. mucosospermus, suggesting additional ancestors beyond cordophanus in the lineage of cultivated watermelon. Our investigation substantially improves the comprehension of watermelon genome diversity, furnishing comprehensive reference genomes for all Citrullus species. This advancement aids in the exploration and genetic enhancement of watermelon using its wild relatives.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319210 | PMC |
| http://dx.doi.org/10.1038/s41588-024-01823-6 | DOI Listing |
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