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Detailed Protocol for Solid-Phase Extraction for Lipidomic Analysis. | LitMetric

Detailed Protocol for Solid-Phase Extraction for Lipidomic Analysis.

Methods Mol Biol

Bone-Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX, USA.

Published: July 2024

AI Article Synopsis

Article Abstract

Developing robust analytical techniques is a vital phase to facilitate understanding the roles and impacts of various omic profilings in cellular functions. The comprehensive analysis of various biological molecules within a biological system requires a precise sample preparation technique. Solid-Phase Extraction (SPE) has proven to be an indispensable method in lipidomic analysis, providing an uncomplicated and user-friendly technique for extraction and purification of lipid components from complex biological matrices. Of all the factors influencing the reliability and success of SPE, column or adsorbent materials, flow rate, and storage conditions are paramount in terms of their significance. In this chapter, we will discuss in detail the SPE steps for lipidomic analysis in biofluid samples (serum and plasma) and muscle tissues.

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Source
http://dx.doi.org/10.1007/978-1-0716-3902-3_15DOI Listing

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