AI Article Synopsis
- Intracellular calcium (Ca) levels can be tracked in live cells using sensitive fluorescent dyes.
- The Gαq protein plays a role in lipid signaling pathways that can be explored through calcium imaging.
- Specific protocols for measuring intracellular Ca in esophageal smooth muscle cells will focus on PEG2-EP1 activity, utilizing ratiometric Fura-2 imaging for quantification and Fluo-4 confocal microscopy for high-resolution images.
Article Abstract
Intracellular Ca can be conveniently monitored by sensitive Ca fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca imaging. Here we describe the protocols to measure intracellular Ca for studying PEG2-EP1 activity in esophageal smooth muscle cells. The ratiometric Fura-2 imaging provides quantitative data, and the Fluo-4 confocal microscopic imaging has high-spatial resolution.
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| http://dx.doi.org/10.1007/978-1-0716-3902-3_7 | DOI Listing |
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