AI Article Synopsis
- * The R-1 gene, which regulates seed color in wheat, encodes a MYB-type transcription factor and can be targeted with specific genetic markers for faster breeding.
- * This chapter explains methods for collecting seeds for germination tests, observing seed color using NaOH staining, and genotyping the R-1 genes with multiplex PCR.
Article Abstract
Seed dormancy is an important trait in cereal breeding, as it prevents preharvest sprouting (PHS). Although seed dormancy is a multifactorial trait, seed color has been demonstrated to be a major dormancy-related factor controlled by few genes. The R-1 gene is a seed color regulator that encodes a MYB-type transcription factor in wheat. A set of genetic markers designed against R-1 can provide a powerful tool for swift wheat breeding. Depth of seed dormancy varies not only among lines but also during seed development in each line. In this chapter, we describe how developmental seeds can be collected to perform germination tests, how seed color can be observed after NaOH staining, and how to genotype wheat R-1 genes using multiplex PCR.
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| http://dx.doi.org/10.1007/978-1-0716-3965-8_4 | DOI Listing |
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