AI Article Synopsis
- Subclinical ketosis (SCK) is a common metabolic disorder affecting cows shortly after giving birth, marked by high ketone body levels without visible symptoms, primarily caused by negative energy balance.
- The study aimed to find specific genetic variants (SNPs) linked to SCK using genome-wide association studies (GWAS) and analyze related gene functions through gene-set enrichment analysis (GSEA).
- Researchers identified 194 SNPs and their associated genes, which are involved in various biological pathways and could inform breeding practices to lower SCK risk in dairy cows.
Article Abstract
Subclinical ketosis (SCK) is a prevalent metabolic disorder that occurs during the transition to lactation period. It is defined as a high blood concentration of ketone bodies (beta-hydroxybutyric acid f ≥ 1.2 mmol/L) within the first few weeks of lactation, and often presents without clinical signs. SCK is mainly caused by negative energy balance (NEB). The objective of this study is to identify single nucleotide polymorphisms (SNPs) associated with SCK using genome-wide association studies (GWAS), and to predict the biological functions of proximal genes using gene-set enrichment analysis (GSEA). Blood samples were collected from 112 Holstein cows between 5 and 18 days postpartum to determine the incidence of SCK. Genomic DNA extracted from both SCK and healthy cows was examined using the Illumina Bovine SNP50K BeadChip for genotyping. GWAS revealed 194 putative SNPs and 163 genes associated with those SNPs. Additionally, GSEA showed that the genes retrieved by Database for Annotation, Visualization, and Integrated Discovery (DAVID) belonged to calcium signaling, starch and sucrose, immune network, and metabolic pathways. Furthermore, the proximal genes were found to be related to germ cell and early embryo development. In summary, this study proposes several feasible SNPs and genes associated with SCK through GWAS and GSEA. These candidates can be utilized in selective breeding programs to reduce the genetic risk for SCK and subfertility in high-performance dairy cows.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11222121 | PMC |
| http://dx.doi.org/10.5187/jast.2023.e97 | DOI Listing |
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