AI Article Synopsis
- Researchers created conjugates of a specific compound (benzothiophene-fused azacyclononyne BT9N-NH2) with fluorescent dyes to visualize azidoglycans inside cells.
- The importance of the cycloalkyne core was shown by comparing the new reagents to existing DBCO- and BCN-dye conjugates.
- To enhance specificity during bioconjugation within cells, the study suggests using the less reactive BT9N-dye reagents instead of the more reactive DBCO- and BCN-dye conjugates.
Article Abstract
Conjugates of benzothiophene-fused azacyclononyne BT9N-NH2 with fluorescent dyes were developed to visualise azidoglycans intracellularly. The significance of the cycloalkyne core was demonstrated by comparing new reagents with DBCO- and BCN-dye conjugates. To reduce non-specificity during intracellular bioconjugation using SPAAC, less reactive BT9N-dye reagents are preferred over highly reactive DBCO- and BCN-dye conjugates.
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| http://dx.doi.org/10.1039/d4ob01032a | DOI Listing |
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Article Synopsis
- Researchers created conjugates of a specific compound (benzothiophene-fused azacyclononyne BT9N-NH2) with fluorescent dyes to visualize azidoglycans inside cells.
- The importance of the cycloalkyne core was shown by comparing the new reagents to existing DBCO- and BCN-dye conjugates.
- To enhance specificity during bioconjugation within cells, the study suggests using the less reactive BT9N-dye reagents instead of the more reactive DBCO- and BCN-dye conjugates.
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