sp. nov., sp. nov., and sp. nov., Isolated from Soil.

Three Gram-stain-positive, aerobic, rod-shaped, and non-motile bacteria, labelled as W11, SW19, and YR1, were isolated from soil, and performed their polyphasic taxonomic investigation. The phylogenetic and 16S rRNA gene sequence analysis showed that strains W11, SW19, and YR1 belonged to the genera , , and , respectively. Strain W11 was closely affiliated with SYSU K20354 (98.1%), strain SW19 showed the closest affiliation with ZW T2_19 (97.0%), and strain YR1 was most closely related to KIS18-7 (98.0%). The genome sizes of strains W11, SW19, and YR1 were 4,181,720 bp, 4,740,677 bp, and 4,228,226 bp, respectively, with DNA G+C contents of 70.5%, 64.2%, and 69.7%, respectively. Average nucleotide identity and digital DNA-DNA hybridization values of W11, SW19, and YR1 with their respective reference species were <79.6% and <23.6%, respectively. The predominant cellular fatty acids detected in strain W11 were anteiso-C, iso-C, and anteiso-C. In strain SW19, they were summed feature 9 (C 10-methyl and/or iso-Cω 9c), anteiso-C, and anteiso-C. Strain YR1 exhibited Cω 9c, C 10-methyl, TBSA, and anteiso-C as its major cellular fatty acids. Overall, the polyphasic taxonomic comparisons indicated that strains W11, SW19, and YR1 represent novel species within the genera , , and , respectively. Accordingly, we propose the names sp. nov., with the type strain W11 (=KCTC 49818 =NBRC 115999), sp. nov., with the type strain SW19 (=KCTC 49860 =NBRC 116108), and sp. nov., with the type strain YR1 (=KCTC 49863 =NBRC 116165).