AI Article Synopsis
- - The study identifies eight monocot chimeric jacalin (MCJ) genes in maize, which are spread unevenly across four chromosomes and have distinct expression patterns.
- - ZmMCJs display similarities in protein structures within their modules and primarily localize on the cell membrane; only some have lectin activity attributed to their jacalin-related lectin domain.
- - The research suggests that ZmMCJ genes are likely involved in the plant's response to environmental stresses, as indicated by their predicted interactions with phytohormones like abscisic acid and jasmonic acid.
Article Abstract
Background: The monocot chimeric jacalins (MCJ) proteins, which contain a jacalin-related lectin (JRL) domain and a dirigent domain (DIR), are specific to Poaceae. MCJ gene family is reported to play an important role in growth, development and stress response. However, their roles in maize have not been thoroughly investigated.
Results: In this study, eight MCJ genes in the maize genome (designated as ZmMCJs) were identified, which displayed unequal distribution across four chromosomes. Phylogenetic relationships between the ZmMCJs were evident through the identification of highly conserved motifs and gene structures. Analysis of transcriptome data revealed distinct expression patterns among the ZmMCJ genes, leading to their classification into four different modules, which were subsequently validated using RT-qPCR. Protein structures of the same module are found to be relatively similar. Subcellular localization experiments indicated that the ZmMCJs are mainly located on the cell membrane. Additionally, hemagglutination and inhibition experiments show that only part of the ZmMCJs protein has lectin activity, which is mediated by the JRL structure, and belongs to the mannose-binding type. The cis-acting elements in the promoter region of ZmMCJ genes predicted their involvement response to phytohormones, such as abscisic acid and jasmonic acid. This suggests that ZmMCJ genes may play a significant role in both biotic and abiotic stress responses.
Conclusions: Overall, this study adds new insights into our understanding of the gene-protein architecture, evolutionary characteristics, expression profiles, and potential functions of MCJ genes in maize.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227246 | PMC |
| http://dx.doi.org/10.1186/s12870-024-05354-4 | DOI Listing |
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Article Synopsis
- - The study identifies eight monocot chimeric jacalin (MCJ) genes in maize, which are spread unevenly across four chromosomes and have distinct expression patterns.
- - ZmMCJs display similarities in protein structures within their modules and primarily localize on the cell membrane; only some have lectin activity attributed to their jacalin-related lectin domain.
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