Here, we present a protocol to quantify interactions among difficult-to-express proteins from Drosophila cells using the select western blot-free tagged-protein interaction (SWFTI) assay. We describe steps for plasmid design, cell plating, protein expression, and immunoprecipitation preparation. We then detail procedures for protein labeling, gel purification, and protein quantification. This protocol offers a fluorescence-based technique for rapid quantification of ectopically expressed proteins that are fused to SNAP and CLIP tags without the need for membrane transfer. For complete details on the use and execution of this protocol, please refer to Lin et al..
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264177 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103171 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Associations between biomarkers of inflammation and depressive symptoms-potential differences between diabetes types and symptom clusters of depression.
Transl Psychiatry
January 2025
German Center for Diabetes Research (DZD), München-Neuherberg, Germany.
Inflammation is a probable biological pathway underlying the relationship between diabetes and depression, but data on differences between diabetes types and symptom clusters of depression are scarce. Therefore, this cross-sectional study aimed to compare associations of a multimarker panel of biomarkers of inflammation with depressive symptoms and its symptom clusters between people with type 1 diabetes (T1D) and type 2 diabetes (T2D). This cross-sectional study combined data from five studies including 1260 participants (n = 706 T1D, n = 454 T2D).
View Article and Find Full Text PDFReplisomal coupling between the α-Pol III core and the τ-subunit of the clamp loader complex (CLC) are essential for genomic integrity in E. coli.
J Biol Chem
January 2025
Department of Chemistry and Biochemistry, Baylor University, Waco, Texas, 76798-7348, USA. Electronic address:
Coupling interactions between the alpha (α) subunit of the polymerase III core (α-Pol III core) and the tau (τ) subunit of the clamp loader complex (τ-CLC) are vital for efficient and rapid DNA replication in Escherichia coli (E. coli). Specific and targeted mutations in the C-terminal τ-interaction region of the Pol III α-subunit disrupted efficient coupled rolling circle DNA synthesis in vitro and caused significant genomic defects in CRISPR-Cas9 dnaE edited cell strains.
View Article and Find Full Text PDFDouble trouble? Quantifying the risk from co-exposure to multiple pathogens in Tenebrio molitor at different CO concentrations.
J Invertebr Pathol
January 2025
UK Centre for Ecology & Hydrology, Maclean Building, Benson Lane, Crowmarsh Gifford, Wallingford, Oxfordshire OX10 8BB, United Kingdom. Electronic address:
The insect mass-rearing industry to produce feed and food is expanding rapidly. Insects in production frequently encounter multiple pathogens and environmental stressors simultaneously, which can lead to significant economic losses. Our understanding of the interactions between different stressors remains limited, and existing methods primarily focus on determining overall patterns of additivity, synergism, or antagonism.
View Article and Find Full Text PDFThe influence of subtidal Laminaria canopies on local environmental conditions and the structure of understorey communities.
Mar Environ Res
January 2025
Marine Biological Association of the United Kingdom, The Laboratory, Citadel Hill, PlySmouth, PL1 2PB, UK. Electronic address:
Understanding the role of species interactions (e.g. competition and facilitation) in structuring communities is a fundamental goal of ecology.
View Article and Find Full Text PDFInfluence of Kunth Flavonoids on Composition of Soil Microbial Community.
Int J Mol Sci
December 2024
Key Laboratory of Agro-Environment in Tropics, Ministry of Agriculture and Rural Affairs, Guangdong Engineering Research Centre for Modern Eco-Agriculture and Circular Agriculture, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
, one of the world's most destructive invasive species, is known for causing significant ecological and economic harm. While extensive research has focused on its growth characteristics, secondary metabolites, and control measures, its chemical interactions with the environment-particularly the role of flavonoids in shaping soil microbial communities-remain underexplored. In this study, we identified and quantified ten flavonoids from root exudates using UPLC-MS, including Hispidulin, Isorhamnetin, and Mikanin.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!