Alpha-1-acid glycoprotein (AGP) is a heterogeneous glycoprotein fulfilling key roles in many biological processes, including transport of drugs and hormones and modulation of inflammatory and immune responses. The glycoform profile of AGP is known to change depending on (patho)physiological states such as inflammatory diseases or pregnancy. Besides complexity originating from five N-glycosylation sites, the heterogeneity of the AGP further expands to genetic variants. To allow in-depth characterization of this intriguing protein, we developed a method using anion exchange chromatography (AEX) coupled to mass spectrometry (MS) revealing the presence of over 400 proteoforms differing in their glycosylation or genetic variants. More precisely, we could determine that AGP mainly consists of highly sialylated higher antennary structures with on average 16 sialic acids and 0 or 1 fucose per protein. Interestingly, a slightly higher level of fucosylation was observed for AGP1 variants compared to that of AGP2. Proteoform assignment was supported by integrating data from complementary MS-based approaches, including AEX-MS of an exoglycosidase-treated sample and glycopeptide analysis after tryptic digestion. The developed analytical method was applied to characterize AGP from plasma of women during and after pregnancy, revealing differences in glycosylation profiles, specifically in the number of antennae, HexHexNAc units, and sialic acids.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232096 | PMC |
| http://dx.doi.org/10.1021/acs.jproteome.4c00107 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
From solubility to efficiency: Per- and polyfluoroalkyl substances (PFAS) regeneration from anion exchange resins.
Sci Total Environ
January 2025
Department of Civil and Environmental Engineering, Temple University, Philadelphia, PA 19122, USA. Electronic address:
This study investigated the regenerability of anion exchange resins for per- and polyfluoroalkyl substances (PFAS), focusing on the interaction between regenerant composition and resin characteristics. The influence of salt type and concentration on PFAS solubility revealed a general decline in perfluorohexane sulfonate (PFHxS) solubility with increased salt concentrations, most strongly with KCl followed by NaCl and NHCl. Mixed solubility results were observed for perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS).
View Article and Find Full Text PDFAtomically Dispersed Co-P Moieties via Direct Thermal Exfoliation for Alkaline Hydrogen Electrosynthesis.
J Am Chem Soc
January 2025
National Center for Nanoscience and Technology, No. 11 ZhongGuanCun BeiYiTiao, Beijing 100190, China.
The development of highly active and stable cathodes in alkaline solutions is crucial for promoting the commercialization of anion exchange membrane (AEM) electrolyzers, yet it remains a significant challenge. Herein, we synthesized atomically dispersed CoP moieties (CoP-SSC) immobilized on ultrathin carbon nanosheets via a phosphidation exfoliation strategy at medium temperature. The thermodynamic formation process of the Co-P moieties was elucidated using X-ray absorption spectroscopy (XAS) and theoretical calculations.
View Article and Find Full Text PDF«Green-Ligand» in Metallodrugs Design-Cu(II) Complex with Phytic Acid: Synthetic Approach, EPR-Spectroscopy, and Antimycobacterial Activity.
Molecules
January 2025
N. S. Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prosp. 31, 119991 Moscow, Russia.
The interaction of sodium phytate hydrate CHOP·xNa·yHO (phytNa) with Cu(OAc)·HO and 1,10-phenanthroline (phen) led to the anionic tetranuclear complex [Cu(HO)(phen)(phyt)]·2Na·2NH·32HO (), the structure of the latter was determined by X-ray diffraction analysis. The phytate is completely deprotonated; six phosphate fragments (with atoms P1-P6) are characterized by different spatial arrangements relative to the cyclohexane ring (1a5e conformation), which determines two different types of coordination to the complexing agents-P1 and P3, P4, and P6 have monodentate, while P2 and P5 are bidentately bound to Cu cations. The molecular structure of the anion complex is stabilized by a set of strong intramolecular hydrogen bonds involving coordinated water molecules.
View Article and Find Full Text PDFSeparation of Inorganic Forms of Tellurium Using On-Site SPE Followed by ICP-MS or ICP-OES-The Right Solution for Water Monitoring.
Molecules
January 2025
Faculty of Chemistry, University of Warsaw, ul. Pasteura 1, 02-093 Warsaw, Poland.
Tellurium, recognized as one of the technology-critical elements, should be considered as a xenobiotic. Its application, i.a.
View Article and Find Full Text PDFVDAC1: A Key Player in the Mitochondrial Landscape of Neurodegeneration.
Biomolecules
December 2024
Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer Sheva 84105, Israel.
Voltage-Dependent Anion Channel 1 (VDAC1) is a mitochondrial outer membrane protein that plays a crucial role in regulating cellular energy metabolism and apoptosis by mediating the exchange of ions and metabolites between mitochondria and the cytosol. Mitochondrial dysfunction and oxidative stress are central features of neurodegenerative diseases. The pivotal functions of VDAC1 in controlling mitochondrial membrane permeability, regulating calcium balance, and facilitating programmed cell death pathways, position it as a key determinant in the delicate balance between neuronal viability and degeneration.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!