Chitosan, a biopolymer obtained from chitin, is known for its remarkable adsorption abilities for dyes, drugs, and fats, and its diverse array of antibacterial characteristics. This study explores the extraction and characterization of chitosan from the mycelium of . The moisture content, ash content, water binding capacity, fat binding capacity, and degree of deacetylation of the extracted chitosan were determined. The chitosan exhibited a high yield of 70%, crystallinity of 49.07%, a degree of deacetylation of 86%, and potent antimicrobial properties against both Gram-negative and Gram-positive bacteria. The study also examined the adsorption capabilities of chitosan to remove methylene blue (MB) dye by analysing specific factors like pH, reaction time, and MB concentration using the response surface model. The highest degree of MB dye removal was 91.6% at a pH of 6, a reaction time of around 60 min and an initial dye concentration of 16 ppm. This experimental design can be applied for chitosan adsorption of other organic compounds such as dyes, proteins, drugs, and fats.
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http://dx.doi.org/10.3389/fchem.2024.1353524 | DOI Listing |
Int J Mol Sci
December 2024
Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Blonie, Poland.
Chitosan (CS), derived from the partial deacetylation and hydrolysis of chitin, varies in the degree of deacetylation, molecular weight, and origin, influencing its biological effects, including antifungal properties. In plants, CS triggers immune responses and stimulates biomass growth. Previously, we found that the antifungal activity of CS was strongly dependent on its physicochemical properties.
View Article and Find Full Text PDFAMB Express
January 2025
Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt.
A Novel cold-active chitin deacetylase from Shewanella psychrophila WP2 (SpsCDA) was overexpressed in Escherichia coli BL21 and employed for deacetylation of chitin to chitosan. The produced chitosan was characterized, and its antifungal activity was investigated against Fusarium oxysporum. The purified recombinant SpsCDA appeared as a single band on SDS-PAGE at approximately 60 kDa, and its specific activity was 92 U/mg.
View Article and Find Full Text PDFFood Chem
December 2024
Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland. Electronic address:
The firmness of the two apple varieties: Idared and Pinova was similar during ripening, while it decreased significantly during 3-month storage only for Idared. Pectin-rich fractions were isolated from apple flesh tissue: water-soluble pectin (WSP), imidazole-soluble pectin (ISP), and hemicellulose-rich fractions: natively acetylated hemicelluloses (LiCl-DMSO), deacetylated hemicelluloses (KOH). It was shown that the degree of acetylation (DAc) of the hemicelluloses fraction (LiCl-DMSO) increased during apple ripening and storage, with higher values for Idared.
View Article and Find Full Text PDFLangmuir
December 2024
Laboratory of Molecular Chemistry, Materials and Environment (LCM2E), Department of Chemistry, Multidisciplinary Faculty of Nador, University Mohamed I, 60700 Nador, Morocco.
This work involves the synthesis of two chitosan derivatives by reacting chitosan, extracted from shrimp shells in eastern Morocco, with 2-nitrobenzaldehyde via a Schiff base reaction. An amino derivative of chitosan was then produced by reducing the imine group created by sodium borohydride. We investigated the molecular weight (), crystallinity index (), and degree of deacetylation () of the isolated chitosan, among other characteristic features.
View Article and Find Full Text PDFMaterials (Basel)
November 2024
Research Center for Complex Physical Systems, Faculty of Sciences, Lucian Blaga University of Sibiu, 550012 Sibiu, Romania.
Chitosan, a natural biopolymer with excellent biocompatibility, biodegradability, and modifiable structure, has broad applications in regenerative medicine, tissue engineering, food packaging, and environmental technology. Its abundance, solubility in acidic solutions, and capacity for chemical modification make it highly adaptable for creating specialized derivatives with enhanced properties. Recent advances have demonstrated chitosan's efficacy in composite systems for tissue regeneration, drug delivery, and antimicrobial applications.
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