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Comparison of shipping versus immediate freezer storage of vaginal samples for vaginal microbiota assessment. | LitMetric

AI Article Synopsis

  • This study examined whether storing vaginal samples at room temperature in stabilizing solutions affects microbiota analysis compared to immediate freezing.
  • Twenty participants collected vaginal swabs using two different nucleic acid preservatives, with some samples being frozen and others shipped for analysis.
  • Results showed that there was complete agreement in microbiota composition between shipped and frozen samples, suggesting that home collection and mailing of samples is viable for research and clinical use.

Article Abstract

Unlabelled: ObjectivesWe evaluated how storing vaginal samples at room temperature in stabilising solutions versus immediate freezing affects 16S rRNA gene amplicon sequencing-based microbiota studies, aiming to simplify home and field collection.

Methods: Twenty participants self-collected six mid-vaginal swabs that were stored in two nucleic acid preservatives (three in modified Solution C2 (Qiagen) and three in Amies/RNALater (Sigma)) in January-February 2016. From each set, two were immediately frozen (-80°C) and one was shipped to the University of Idaho (Moscow, Idaho) with return shipping to the Institute for Genome Sciences (Baltimore, Maryland). Amplicon sequencing of the 16S rRNA gene was used to characterise the vaginal microbiota, VALENCIA was used to assign community state types (CSTs), and quantitative PCR (qPCR) of 16S rRNA genes was used to estimate bacterial abundance. Cohen's Kappa statistic was used to assess within-participant agreement. Bayesian difference of means models assessed within-participant comparisons between shipped and immediately frozen samples.

Results: There were 115 samples available for analysis. Average duration of transit for shipped samples was 8 days (SD: 1.60, range: 6-11). Within-participant comparisons of CSTs between shipped and immediately frozen samples revealed complete concordance (kappa: 1.0) for both preservative solutions. No significant differences comparing shipped and immediately frozen samples were found with taxon-level comparisons or bacterial abundances based on pan-bacterial qPCR.

Conclusions: Short-term room temperature shipping of vaginal swabs placed in stabilising solutions did not affect vaginal microbiota composition. Home collection with mail-in of vaginal samples may be a reasonable approach for research and clinical purposes to assess the vaginal microbiota.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11356182PMC
http://dx.doi.org/10.1136/sextrans-2023-056100DOI Listing

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