Quantum dots (QDs), with their unique optical and physical properties, have revolutionized the field of biological imaging, providing researchers with tools to explore cellular processes and molecular interactions in unprecedented detail. This review explores the diverse properties of QDs, emphasizing their application in biological imaging and addressing both their advantages and challenges. We discuss the developments in QD technology that have facilitated their integration into bioimaging, highlighting the role of surface modifications in enhancing their biocompatibility and functionality. The varied applications of QDs in both and imaging settings are examined, showcasing their capacity to deliver brighter, more stable, and multiplexed imaging solutions compared to traditional fluorescent dyes. Furthermore, we delve into the challenges associated with QD use, particularly concerns regarding their potential toxicity and long-term effects on biological systems, and explore ongoing research aimed at mitigating these issues. Finally, we discuss future directions in QD technology, anticipating advancements that will further solidify their role in biological imaging and open up new avenues for scientific exploration.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11217725PMC
http://dx.doi.org/10.1039/d4ra04402aDOI Listing

Publication Analysis

Top Keywords

biological imaging
16
field biological
8
imaging
6
biological
5
utilization quantum
4
quantum dot
4
dot labeling
4
labeling burgeoning
4
burgeoning technique
4
technique field
4

Similar Publications

4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos.

STAR Protoc

January 2025

Gladstone Institutes, San Francisco, CA, USA; Roddenberry Center for Stem Cell Biology and Medicine at Gladstone, San Francisco, CA, USA; Department of Pediatrics, Cardiovascular Research Institute, Institute for Human Genetics, and Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address:

As light sheet fluorescence microscopy (LSFM) becomes widely available, reconstruction of time-lapse imaging will further our understanding of complex biological processes at cellular resolution. Here, we present a comprehensive workflow for in toto capture, processing, and analysis of multi-view LSFM experiments using the ex vivo mouse embryo as a model system of development. Our protocol describes imaging on a commercial LSFM instrument followed by computational analysis in discrete segments, using open-source software.

View Article and Find Full Text PDF

Purpose Of Review: This review aims to evaluate current diagnostic and therapeutic strategies for postpericardiotomy syndrome (PPS), with a focus on the evolving role of multimodality imaging, including echocardiography, cardiac computed tomography (CCT), and cardiac magnetic resonance imaging (CMR). The review also explores the potential benefits of advanced imaging in improving the accuracy and management of PPS.

Recent Findings: PPS, a common complication following cardiac surgery, presents with pleuritic chest pain, fever, and pericardial or pleural effusion.

View Article and Find Full Text PDF

Chiral Nanostructures from Artificial Helical Polymers: Recent Advances in Synthesis, Regulation, and Functions.

ACS Nano

January 2025

School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen (CUHK-Shenzhen), Guangdong 518172, P.R. China.

Helical structures such as right-handed double helix for DNA and left-handed α-helix for proteins in biological systems are inherently chiral. Importantly, chirality at the nanoscopic level plays a vital role in their macroscopic chiral functionalities. In order to mimic the structures and functions of natural chiral nanoarchitectures, a variety of chiral nanostructures obtained from artificial helical polymers are prepared, which can be directly observed by atomic force microscopy (AFM), scanning tunneling microscopy (STM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).

View Article and Find Full Text PDF

Aims: Human periodontal ligament stem cells (hPDLSCs) exhibit an enormous potential to regenerate periodontal tissue. However, their translatability to the clinical setting is constrained by technical difficulties in standardizing culture conditions. The aim was to assess complex culture conditions using a proteomic-based protocol to standardize multi-layer hPDLSC cultivation methodology.

View Article and Find Full Text PDF

Inflammation, a central process in numerous diseases, plays a crucial role in hepatic disorders, arthritis, cardiac conditions, and neurodegenerative ailments. Given the lack of effective anti-inflammatory drugs, it is imperative to assess inflammation severity and explore novel therapeutics. Selenocysteine (Sec), a key mediator of selenium's biological function, is closely involved in anti-inflammatory responses.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!