AI Article Synopsis
- Epstein-Barr virus (EBV) is linked to various cancers and diseases, and its DNA detection is crucial for diagnosis and prognosis.
- The study evaluated the effectiveness of detecting EBV DNA from dried blood spots (DBS) compared to traditional whole blood samples, finding that while DBS showed 100% specificity, sensitivity varied depending on viral load.
- Results demonstrated that as the EBV DNA load increased, the detection sensitivity in DBS improved, and there was a good correlation between EBV levels in whole blood and DBS, indicating DBS as a viable option for EBV diagnosis and monitoring.
Article Abstract
Epstein-Barr virus (EBV) is a ubiquitous and oncogenic virus that is associated with various malignancies and non-malignant diseases and EBV DNA detection is widely used for the diagnosis and prognosis prediction for these diseases. The dried blood spots (DBS) sampling method holds great potential as an alternative to venous blood samples in geographically remote areas, for individuals with disabilities, or for newborn blood collection. Therefore, the objective of this study was to assess the viability of detecting EBV DNA load from DBS. Matched whole blood and DBS samples were collected for EBV DNA extraction and quantification detection. EBV DNA detection in DBS presented a specificity of 100 %. At different EBV DNA viral load in whole blood, the sensitivity of EBV DNA detection in DBS was 38.78 % (≥1 copies/mL), 43.18 % (≥500 copies/mL), 58.63 % (≥1000 copies/mL), 71.43 % (≥2000 copies/mL), 82.35 % (≥4000 copies/mL), and 92.86 % (≥5000 copies/mL), respectively. These results indicated that the sensitivity of EBV DNA detection in DBS increased with elevating viral load. Moreover, there was good correlation between EBV DNA levels measured in whole blood and DBS, and on average, the viral load measured in whole blood was about 6-fold higher than in DBS. Our research firstly demonstrated the feasibility of using DBS for qualitative and semi-quantitative detection of EBV DNA for diagnosis and surveillance of EBV-related diseases.
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| http://dx.doi.org/10.1016/j.jcv.2024.105710 | DOI Listing |
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