Analysis of cGMP Signaling in Dictyostelium.

Methods Mol Biol

Department of Cell Biochemistry, University of Groningen, Groningen, The Netherlands.

Published: July 2024

Biochemical assays are described to analyze signal transduction by the second messenger cGMP in Dictyostelium. The methods include enzyme assays to measure the activity and regulation of cGMP synthesizing guanylyl cyclases and cGMP-degrading phosphodiesterases. In addition, several methods are described to quantify cGMP levels. The target of cGMP in Dictyostelium is the large protein GbpC that has multiple domains including a Roc domain, a kinase domain, and a cGMP-stimulated Ras-GEF domain. A cGMP-binding assay is described to detect and quantify GbpC.

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http://dx.doi.org/10.1007/978-1-0716-3894-1_13DOI Listing

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