sp. nov. and sp. nov., isolated from soil and faeces of Tibetan antelope () on the Qinghai-Tibet Plateau.

Two novel strain pairs (HM61/HM23 and S-34/S-58) were isolated from soil and the faeces of Tibetan antelope () collected at the Qinghai-Tibet Plateau of PR China. All four new isolates were aerobic, non-motile, Gram-stain-positive, catalase-positive, oxidase-negative, and short rod-shaped bacteria. The results of phylogenetic analysis based on the full-length 16S rRNA genes and 283 core genomic genes indicated that the four strains were separated into two independent branches belonging to the genus . Strains HM61 and HM23 were most closely related to THG T63 (98.58 and 98.65 % 16S rRNA gene sequence similarity). Strains S-34 and S-58 were most closely related to MMS20-HV4-12 (98.89 and 98.89 % 16S rRNA gene sequence similarity). The G+C contents of the genomic DNA of strains HM61 and S-34 were 70.6 and 72.5 mol%, respectively. Strains HM61, S-34 and the type strains of closely related species in the analysis had average nucleotide identity values of 75.4-90.5 % as well as digital DNA-DNA hybridization values between 20.1 and 40.8 %, which clearly indicated that the four isolates represent two novel species within the genus . The chemotaxonomic characteristics of strains HM61 and S-34 were consistent with the genus . The major fatty acids of all four strains were -C, C 8 or C 9. For strains HM61 and S-34, MK-8(H) was the predominant respiratory quinone, ll-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, and the polar lipids profiles were composed of diphosphatidylglycerol and phosphatidylglycerol. Based on phylogenetic, phenotypic, and chemotaxonomic data, we propose that strains HM61 and S-34 represent two novel species of the genus , respectively, with the names sp. nov. and sp. nov. The type strains are HM61 (=GDMCC 4.343=JCM 36399) and S-34 (=CGMCC 4.7664=JCM 33792).