The success of using the insect cell-baculovirus expression technology (BEST) relies on the efficient construction of recombinant baculovirus with genetic stability and high productivity, ideally within a short time period. Generation of recombinant baculoviruses requires the transfection of insect cells, harvesting of recombinant baculovirus pools, isolation of plaques, and the expansion of baculovirus stocks for their use for recombinant protein production. Moreover, many options exist for selecting the genetic elements to be present in the recombinant baculovirus. This chapter describes the most commonly used homologous recombination systems for the production of recombinant baculoviruses, as well as strategies to maximize generation efficiency and recombinant protein or baculovirus production. The key steps for generating baculovirus stocks and troubleshooting strategies are described.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-3961-0_2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins.
Vet Q
December 2025
Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Senecavirus A (SVA) is the causative agent associated with porcine idiopathic vesicular disease (PIVD), a condition indistinguishable from other foreign vesicular diseases affecting pigs. This complicates differential diagnosis and impacts the global swine industry. A diagnostic ELISA based on a non-structural viral protein has been developed, capable of distinguishing infected from vaccinated animals (DIVA).
View Article and Find Full Text PDFProtective humoral immunity induced by virus-like particles expressing Toxoplasma gondii CST1 or MIC8.
Acta Trop
January 2025
Department of Medical Zoology, School of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea; Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Re-search Institute (CRI), Kyung Hee University, Seoul, 02447, Republic of Korea. Electronic address:
Protective efficacy assessment of toxoplasmosis vaccines, at least at the preclinical level, frequently involves lethal dose challenge infection. Nonetheless, their efficacies remain largely unexplored against low infection doses which better reflects how humans become infected in the real world. In this study, we compared the immunity elicited in mice that were heterologously immunized with recombinant baculovirus and virus-like particles expressing either the cyst wall protein (CST1) or microneme protein 8 (MIC8) of Toxoplasma gondii (T.
View Article and Find Full Text PDFRole of ubiquitin-proteasome pathway in budded virus egress and GP64 surface distribution in Bombyx mori nucleopolyhedrovirus.
J Gen Virol
December 2024
Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, PR China.
The Bombyx mori nucleopolyhedrovirus (BmNPV) is a DNA virus that affects the silkworm, , causing substantial economic losses in sericulture. This study investigates the mechanisms underlying budded virus egress, focusing on the roles of the ubiquitin-proteasome pathway (UPP) machinery. BmNPV produces two virion types: budded virions (BVs) and occlusion-derived virions (ODVs), which differ in their envelope origins and functions.
View Article and Find Full Text PDFA novel digestive protease chymotrypsin-like serine contributes to anti-BmNPV activity in silkworm (Bombyxmori).
Dev Comp Immunol
January 2025
Anhui Province Key Laboratory of Resource Insect Biology and Innovative Utilization, School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China; Anhui International Joint Research and Developmental Center of Sericulture Resources Utilization, Hefei, 230036, China. Electronic address:
Serine proteases (SPs) are important proteases in the digestive system of lepidopteran insects. They play important roles in protein digestion, coagulation, signal transduction, hormone activation, inflammation and development. Blood-borne pyosis caused by Bombyx mori nuclear polyhedrosis virus (BmNPV) has caused serious harm to sericulture.
View Article and Find Full Text PDFConstruction with recombinant epitope-expressing baculovirus enhances protective effects of inactivated H9N2 vaccine against heterologous virus.
Vet Microbiol
January 2025
National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China; UK-China Centre of Excellence for Research on Avian Diseases, Guangzhou 510642, PR China. Electronic address:
Article Synopsis
- - The study aimed to improve the effectiveness of inactivated H9N2 avian influenza vaccines by combining them with a recombinant baculovirus containing multiple T and B cell epitopes, showing better immune response than using the vaccine alone.
- - Results indicated that the combined immunization led to significantly higher levels of antibodies and T cell responses, particularly after exposure to a heterologous virus, which suggests a robust immune boost.
- - The InV+BV-BNT group showed lower viral loads and virus positivity rates in samples taken after infection, highlighting its potential as a supplementary vaccine to enhance protection against H9N2 avian influenza.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!