The substantial repertoire of laser radiation--its coherence, range of intensity and frequency, controlability of focal area and of beam length--has been comprehensively explored in the contexts of micro- and macro-diagnostics of cells, biochemical kinetics, therapy and surgery.
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Exploring Environmental Microfungal Diversity Through Serial Single Cell Screening.
Mol Ecol Resour
January 2025
Chair of Urban Water Systems Engineering, Technical University of Munich, Garching, Germany.
Known for its remarkable diversity and ecological importance, the fungal kingdom remains largely unexplored. In fact, the number of unknown and undescribed fungi is predicted to exceed the number of known fungal species by far. Despite efforts to uncover these dark fungal taxa, we still face inherent sampling biases and methodological limitations.
View Article and Find Full Text PDFOnline integration of capillary electrophoresis and dual detector Taylor dispersion analysis a 3D printed instrument.
Analyst
January 2025
Department of Chemistry & Biochemistry, New Mexico State University, Las Cruces, NM, 88003-001, USA.
Hydrodynamic radius () is a descriptive metric of protein structure with the potential to impact drug development, disease diagnosis, and other important research areas of molecular biology. Common instrumental methods for molecular size characterization are disadvantageous due to high sample consumption, measurements made in non-physiological conditions, and/or inaccurate size determinations. Capillary Taylor dispersion analysis (TDA) is a molecular sizing method that utilizes nL sample volumes and achieves absolute size determination without calibration or comparison to standards.
View Article and Find Full Text PDFMinimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy.
Front Neuroanat
January 2025
Experimental Research Centre for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, Craiova, Romania.
Background: While widefield microscopy has long been constrained by out-of-focus scattering, advancements have generated a solution in the form of confocal laser scanning microscopy (cLSM) and optical sectioning microscopy using structured illumination (OSM). In this study, we aim to investigate, using microglia branching, if cLSM and OSM can produce images with comparable morphological characteristics.
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Selective toxicity of a novel antimicrobial peptide Acidocin 4356 against Pseudomonas aeruginosa and Acinetobacter baumannii in human cell-based in vitro infection models.
Sci Rep
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Department of Energy & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
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View Article and Find Full Text PDFUnsaturated Fatty Acids Are Decreased in Aβ Plaques in Alzheimer's Disease.
J Neurochem
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Center for Protein Diagnostics (PRODI) Biospectroscopy, Ruhr University Bochum, Bochum, Germany.
Alzheimer's disease (AD) is characterized by the accumulation of amyloid-beta (Aβ) plaques in the brain, contributing to neurodegeneration. This study investigates lipid alterations within these plaques using a novel, label-free, multimodal approach. Combining infrared (IR) imaging, machine learning, laser microdissection (LMD), and flow injection analysis mass spectrometry (FIA-MS), we provide the first comprehensive lipidomic analysis of chemically unaltered Aβ plaques in post-mortem human AD brain tissue.
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