Metagenomic sequences from anaerobic chloroform and dichloromethane degrading microbial communities.

Microbiol Resour Announc

Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.

Published: July 2024

Here, we present metagenomes from two cultures derived from an anaerobic microbial consortium used for bioremediation. One culture dechlorinates chloroform to dichloromethane, which is further mineralized to CO. A second subculture was amended with only dichloromethane. We sought draft genomes of key microorganisms to identify metabolic potential in these consortia.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11324032PMC
http://dx.doi.org/10.1128/mra.00391-24DOI Listing

Publication Analysis

Top Keywords

chloroform dichloromethane
8
metagenomic sequences
4
sequences anaerobic
4
anaerobic chloroform
4
dichloromethane degrading
4
degrading microbial
4
microbial communities
4
communities metagenomes
4
metagenomes cultures
4
cultures derived
4

Similar Publications

An exploratory study of volatile and semi-volatile organic compounds in PM atmospheric particles from an outdoor environment in Brazil.

Environ Sci Pollut Res Int

December 2024

Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, Belo Horizonte, MG, 6627, 370901, Brazil.

The development of methods for determining volatile and semi-volatile organic compounds in public spaces has become necessary to identify potential health and environmental risks. This study presents a practical methodology for sampling, extracting, detecting, and identifying these compounds in a vehicular traffic region in Belo Horizonte, Brazil. The methodology uses direct-immersion solid phase microextraction (DI-SPME) and static headspace (SHS) to extract SVOCs/VOCs.

View Article and Find Full Text PDF

Efficient removal of lipophilic compounds from sewage sludge: Comparative evaluation of solvent extraction techniques.

Heliyon

December 2024

Water Systems and Biotechnology Institute, Faculty of Natural Sciences and Technology, Riga Technical University, Kipsalas 6a, Riga, Latvia.

Municipal sewage sludge, a by-product of wastewater treatment plants, presents environmental challenges due to its complex composition. Particular concern is the lipophilic and aliphatic compounds that pose risks to the environment and human health. This study focuses on the efficient removal of those compounds from sewage sludge using several organic solvents (hexane, toluene, chloroform, dichloromethane, acetone, hexane-methanol mixture, ethanol, and methanol) and ionic liquids (ILs) like tetrakis(hydroxymethyl)phosphonium chloride and 1-ethyl-3-methylimidazolium acetate by solvent extraction techniques.

View Article and Find Full Text PDF

An Ion Pair Receptor for Selective Solid-Liquid Extraction of LiCl.

Chemistry

December 2024

Department of Chemistry and Research Institute of Natural Science, Gyeongsang National University, Jinju, 52828, Korea.

We have synthesized a new ion pair receptor (2) consisting of a cone-calix[4]arene bearing a combination of ethyl esters and ethers as a cation binding site and a calix[4]pyrrole subunit acting as an anion binding site. Ion pair receptor 2 complexes LiCl, NaCl, and CsCl among alkali metal chloride salts with distinct complexation modes and affinities in 10 % methanol-d in chloroform-d. For instance, relatively small Li and Na cations are bound to the oxygen atoms of the functionalized calix[4]arene lower rim with Cl being hydrogen bonded to the calix[4]pyrrole NHs while the larger Cs cation is bound to the cone-shaped calix[4]pyrrole cavity via a π-cation interaction.

View Article and Find Full Text PDF

The study on (Orange Jasmine) stem bark extract found it to have antioxidant and cytotoxic proper-ties. The structures of the isolated phytoconstituents were determined using NMR spectroscopy. Compounds were evaluated for their potential to be aldose reductase inhibitors using molecular docking and dynamics (MD) simulations.

View Article and Find Full Text PDF

Pangenomic insights into evolution and acquisition of functional genes for bioremediation.

Microb Genom

November 2024

Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.

is a genus of organohalide-respiring bacteria that is recognized for its fastidious growth using reductive dehalogenases (RDases). In the SC05 culture, however, a population also mineralizes dichloromethane (DCM) produced by chloroform dechlorination using the cassette, just downstream of its active RDase. A closed genome of this DCM-mineralizing lineage has previously evaded assembly.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!