Here, we present metagenomes from two cultures derived from an anaerobic microbial consortium used for bioremediation. One culture dechlorinates chloroform to dichloromethane, which is further mineralized to CO. A second subculture was amended with only dichloromethane. We sought draft genomes of key microorganisms to identify metabolic potential in these consortia.
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http://dx.doi.org/10.1128/mra.00391-24 | DOI Listing |
Environ Sci Pollut Res Int
December 2024
Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, Belo Horizonte, MG, 6627, 370901, Brazil.
The development of methods for determining volatile and semi-volatile organic compounds in public spaces has become necessary to identify potential health and environmental risks. This study presents a practical methodology for sampling, extracting, detecting, and identifying these compounds in a vehicular traffic region in Belo Horizonte, Brazil. The methodology uses direct-immersion solid phase microextraction (DI-SPME) and static headspace (SHS) to extract SVOCs/VOCs.
View Article and Find Full Text PDFHeliyon
December 2024
Water Systems and Biotechnology Institute, Faculty of Natural Sciences and Technology, Riga Technical University, Kipsalas 6a, Riga, Latvia.
Municipal sewage sludge, a by-product of wastewater treatment plants, presents environmental challenges due to its complex composition. Particular concern is the lipophilic and aliphatic compounds that pose risks to the environment and human health. This study focuses on the efficient removal of those compounds from sewage sludge using several organic solvents (hexane, toluene, chloroform, dichloromethane, acetone, hexane-methanol mixture, ethanol, and methanol) and ionic liquids (ILs) like tetrakis(hydroxymethyl)phosphonium chloride and 1-ethyl-3-methylimidazolium acetate by solvent extraction techniques.
View Article and Find Full Text PDFChemistry
December 2024
Department of Chemistry and Research Institute of Natural Science, Gyeongsang National University, Jinju, 52828, Korea.
We have synthesized a new ion pair receptor (2) consisting of a cone-calix[4]arene bearing a combination of ethyl esters and ethers as a cation binding site and a calix[4]pyrrole subunit acting as an anion binding site. Ion pair receptor 2 complexes LiCl, NaCl, and CsCl among alkali metal chloride salts with distinct complexation modes and affinities in 10 % methanol-d in chloroform-d. For instance, relatively small Li and Na cations are bound to the oxygen atoms of the functionalized calix[4]arene lower rim with Cl being hydrogen bonded to the calix[4]pyrrole NHs while the larger Cs cation is bound to the cone-shaped calix[4]pyrrole cavity via a π-cation interaction.
View Article and Find Full Text PDFJ Biomol Struct Dyn
December 2024
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh.
The study on (Orange Jasmine) stem bark extract found it to have antioxidant and cytotoxic proper-ties. The structures of the isolated phytoconstituents were determined using NMR spectroscopy. Compounds were evaluated for their potential to be aldose reductase inhibitors using molecular docking and dynamics (MD) simulations.
View Article and Find Full Text PDFMicrob Genom
November 2024
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.
is a genus of organohalide-respiring bacteria that is recognized for its fastidious growth using reductive dehalogenases (RDases). In the SC05 culture, however, a population also mineralizes dichloromethane (DCM) produced by chloroform dechlorination using the cassette, just downstream of its active RDase. A closed genome of this DCM-mineralizing lineage has previously evaded assembly.
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