Effect of Microfluidization on the Volatiles and Antibacterial, Antifungal, and Cytotoxic Activities of Algerian L. Oil-Loaded Nanoemulsions: and Study.

This study aimed to increase the stability and solubility of the Algerian L. (ASHO) essential oil through nanoencapsulation. Nanoemulsions of ASHO (MF-ASHEO) were developed to evaluate their antioxidant and antimicrobial potential, stability, and cytotoxicity using microfluidization at 150 MPa for five cycles. MF-ASHO showed 8 compounds (99.56%) vs ASHEO's 26 compounds (95.46%). Carvacrol increased to 94.51%, replacing γ-terpinene, which decreased to 0.43%. The MF-ASHEO nanoemulsion had a mean particle size of 41.72 nm, a monomodal size distribution pattern, a mean ζ-potential of -39.4 mV, and a polydispersity index (PDI) mean value of 0.291. Micrographs showed spherical nanoparticles with varying diameters in nm. ASHEO was more toxic than MF-ASHEO against HepG2, Vero, and WI-38, according to the MTT and WST-1 assays. ASHEO demonstrated antiradical and antibacterial activity and inhibited biofilm formation. It also had an enhanced antifungal effect and reduced mycotoxin production. The MF-ASHEO sample showed no activity except in reducing mycotoxin production, where it performed better than ASHEO. and ADME results confirmed the inhibitory action of carvacrol on the key enzymes of the aflatoxin biosynthetic mechanism and the target proteins associated with bactericidal/bacteriostatic effects. The microfluidization process dramatically affects not only the oil's volatile content but also its biological activity.