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Effect of β-hydroxybutyrate acid on gene expression levels of antioxidant biomarkers and growth hormone-related genes in liver cell culture. | LitMetric

AI Article Synopsis

  • The study investigates how oxidative stress in dairy cattle affects diseases and reproduction by examining the role of β-hydroxybutyric acid (BHBA) on antioxidant biomarkers and gene expressions in bovine liver cells.
  • It evaluates four antioxidant biomarkers (MDA, SOD, CAT, GSH Px) using ELISA kits and measures gene expression levels via reverse transcriptase-PCR for various growth-related genes.
  • Results show that higher BHBA concentrations led to lower activities of antioxidant biomarkers and altered gene expressions, indicating BHBA downregulates key genes related to growth hormone and antioxidant defenses in liver cells.

Article Abstract

Introduction: In dairy cattle, oxidative stress is a predominant problem associated with diseases and reproductive health issues. This study aimed to detect the variation in the antioxidant biomarkers by adding different concentrations of β-hydroxybutyric acid (BHBA) and sought to elucidate its effects on the gene expression levels of growth hormone (GH) and antioxidant biomarkers in bovine hepatocytes.

Material And Methods: Four antioxidant biomarkers, namely malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH Px) were evaluated using commercially available bovine ELISA kits. The expression levels of the bovine GH, its receptor (GHR), insulin-like growth factor (IGF), IGF-1, IGF-1 receptor, CAT, SOD, GSH-Px and β-actin (as a reference) genes in liver cell culture were determined by reverse transcriptase-PCR assay.

Results: With the increase of BHBA concentration and culture time, the activities of SOD, CAT, and GSH Px biomarkers in hepatocytes decreased. However, the content of MDA in hepatocytes increased gradually with the increase of hepatocyte culture time and BHBA concentration. The qPCR results revealed that after adding BHBA, gene expression levels of GSH-Px, SOD and IGF biomarkers in hepatocytes began to differ in the culture groups at 12 h, whereas the gene expression level of the CAT and GHR biomarkers in hepatocytes began to differ at 6 h.

Conclusion: Quantitative PCR results showed that the BHBA significantly downregulated the expression levels of the GHR gene and CAT, GSH Px and SOD antioxidant biomarker genes.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210367PMC
http://dx.doi.org/10.2478/jvetres-2024-0037DOI Listing

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