The selective precipitation of low-density lipoproteins (LDL) with polyvinyl sulfate (PVS), and the immunoprecipitation of high-density lipoproteins (HDL) and very-low-density lipoproteins (VLDL) with an anti-HDL antibody, can both be used to establish simple methods for the determination of LDL cholesterol. Whereas the PVS method requires the calculation of LDL cholesterol as the difference of total and supernatant cholesterol, the immunoprecipitation method allows the direct measurement of LDL cholesterol in the supernatant. As a first step, both methods were optimized to yield accurate values for normolipemic and slightly hyperlipemic serum samples. Moreover, the determination of LDL-cholesterol in lipemic sera can be achieved by a combination of immunoprecipitation and polyanion precipitation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0009-9120(85)80093-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Delays in Blood Work and Disease Burden: A Cross-Sectional Analysis of Unmet Blood Work Need and Seven Key Health Conditions Across 21 Countries.
Int J Public Health
January 2025
Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, United States.
Objectives: This study analyzes survey data across 21 countries to explore correlations between delays in blood testing and the prevalence of seven health conditions: thalassaemias, sickle cell disorders, malaria, HIV, high fasting plasma glucose, impaired kidney function, and high LDL cholesterol.
Methods: We analyzed Pandemic Recovery Survey data via multivariable logistic regression to compare blood test delays between individuals with and without medical conditions, while adjusting for sociodemographic factors. We also examined the disease burden using disability-adjusted life years (DALYs) and summary exposure values (SEV) rates.
The effects of low-carbohydrate diet on glucose and lipid metabolism in overweight or obese patients with T2DM: a meta-analysis of randomized controlled trials.
Front Nutr
January 2025
National Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
Background: The dual burden of Type 2 Diabetes Mellitus (T2DM) and obesity is a critical public health issue. Low-carbohydrate diets have emerged as a potential intervention, yet clinical evidence remains inconclusive.
Purpose: This meta-analysis assesses the impact of low-carbohydrate diets on metabolic profiles in overweight or obese T2DM patients, aiming to guide clinical practice.
Application of fourier transform infrared vibrational spectroscopy in identifying early biochemical changes in lipid profiles of individuals undergoing Roux-en-y gastric bypass.
BMC Surg
January 2025
Postgraduate Program in Nutrition and Health, Health Sciences Center, Federal University of Espírito Santo, Av. Mal. Campos, 1468 - Maruípe, Vitória, 29047-100, ES, Brazil.
Background: Fourier transform infrared spectroscopy (FTIR) is an analytical technique increasingly applied in biological analysis. This study investigates the application of FTIR to identify early biochemical changes, particularly in lipid profiles, in individuals undergoing Roux-en-Y gastric bypass (RYGB).
Methods: An observational study involving patients from a university hospital's Bariatric and Metabolic Surgery Program, with evaluations performed before (T0) and two months after (T1) RYGB.
Association of non-highdensity lipoprotein cholesterol to highdensity lipoprotein cholesterol ratio (NHHR) and subsequent hypertension and heart diseases: findings from the CHARLS cohort.
Aging Clin Exp Res
January 2025
Department of Cardiology, Tianjin First Center Hospital, Tianjin, 300192, China.
Purpose: NHHR, the ratio of non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol, is a novel lipid marker associated with the risk of heart diseases and various health conditions. However, there is limited evidence regarding the relationship between NHHR and the onset of hypertension and heart diseases. The aim of this study was to investigate the association between NHHR and the new-onset hypertension and heart diseases among the Chinese middle-aged and older general population.
View Article and Find Full Text PDFImpact of protein intake from a caloric-restricted diet on liver lipid metabolism in overweight and obese rats of different sexes.
Sci Rep
January 2025
Department of Cuisine and Nutrition, School of Tourism and Cuisine, Yangzhou University, Yangzhou, China.
In addition to being linked to an excess of lipid accumulation in the liver, being overweight or obese can also result in disorders of lipid metabolism. There is limited understanding regarding whether different levels of protein intake within an energy-restricted diet affect liver lipid metabolism in overweight and obese rats and whether these effects differ by gender, despite the fact that both high protein intake and calorie restriction can improve intrahepatic lipid. The purpose of this study is to explore the effects and mechanisms of different protein intakes within a calorie-restricted diet on liver lipid metabolism, and to investigate whether these effects exhibit gender differences.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!