Outer membrane vesicles from genetically engineered serovar Typhimurium presenting antigens UreB and CagA induce protection against infection in mice.

causes globally prevalent infections that are highly related to chronic gastritis and even development of gastric carcinomas. With the increase of antibiotic resistance, scientists have begun to search for better vaccine design strategies to eradicate colonization. However, while current strategies prefer to formulate vaccines with a single antigen, their potential has not yet been fully realized. Outer membrane vesicles (OMVs) are a potential platform since they could deliver multiple antigens. In this study, we engineered three crucial antigen proteins (UreB, CagA, and VacA) onto the surface of OMVs derived from serovar Typhimurium (. Typhimurium) mutant strains using the hemoglobin protease (Hbp) autotransporter system. In various knockout strategies, we found that OMVs isolated from the Δ Δ Δ Δ mutants could cause distinct increases in immunoglobulin G (IgG) and A (IgA) levels and effectively trigger T helper 1- and 17-biased cellular immune responses, which perform a vital role in protecting against . Next, OMVs derived from Δ Δ Δ Δ mutants were used as a vector to deliver different combinations of antigens. The antibody and cytokine levels and challenge experiments in mice model indicated that co-delivering UreB and CagA could protect against and antigen-specific T cell responses. In summary, OMVs derived from the . Typhimurium Δ Δ Δ Δ mutant strain as the vector while importing UreB and CagA as antigenic proteins using the Hbp autotransporter system would greatly benefit controlling infection.