Single-cell RNA sequencing is increasingly used to investigate cross-species differences driven by gene expression and cell-type composition in plants. However, the frequent expansion of plant gene families due to whole-genome duplications makes identification of one-to-one orthologues difficult, complicating integration. Here we demonstrate that coexpression can be used to trim many-to-many orthology families down to identify one-to-one gene pairs with proxy expression profiles, improving the performance of traditional integration methods and reducing barriers to integration across a diverse array of plant species.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11266096 | PMC |
| http://dx.doi.org/10.1038/s41477-024-01738-4 | DOI Listing |
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