AI Article Synopsis
- Epigenetic processes impact health by changing gene expression without altering the DNA sequence, involving marks like DNA modifications and protein alterations.
- Histone methylation serves as a key example of how these epigenetic marks can activate or silence genes, influenced by their specific placement and intensity.
- A new isotopically labeled version of S-adenosyl-L-methionine (CD-BrSAM) was created to specifically track the activity of the histone methyltransferase DOT1L using mass spectrometry, which could help investigate other methyltransferases as well.
Article Abstract
Epigenetic processes influence health and disease through mechanisms which alter gene expression. In contrast to genetic changes which affect DNA sequences, epigenetic marks include DNA base modifications or post-translational modification (PTM) of proteins. Histone methylation is a prominent and versatile example of an epigenetic marker: gene expression or silencing is dependent on the location and extent of the methylation. Protein methyltransferases exhibit functional redundancy and broad preferences for multiple histone residues, which presents a challenge for the study of their individual activities. We developed an isotopically labelled analogue of co-factor S-adenosyl-L-methionine (CD-BrSAM), with selectivity for the histone lysine methyltransferase DOT1L, permitting tracking of methylation activity by mass spectrometry (MS). This concept could be applied to other methyltransferases, linking PTM discovery to enzymatic mediators.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211345 | PMC |
| http://dx.doi.org/10.1038/s42004-024-01227-x | DOI Listing |
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