AI Article Synopsis
- Food allergens cause abnormal immune responses in sensitive individuals, highlighting the need for effective detection methods.
- A new fluorescent aptasensor using CRISPR technology has been developed, leveraging the unique recognition ability of aptamers and the targeted cutting action of Cas12a to enhance sensitivity and reduce interference.
- Testing showed that this aptasensor can detect food allergens, like lysozyme, with a low detection limit and strong selectivity, paving the way for improved food allergen detection methods.
Article Abstract
Food allergens elicit abnormal immune system responses among allergic individuals and sensitive detection for allergenic ingredient is greatly significant. To address this need, a novel fluorescent aptasensor, assisted by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), have been developed for food allergens. In this study, aptamer offers distinctive recognition capabilities in binding specific targets, while CRISPR-associated-12a protein (Cas12a) holds precise cis-cleavage for cutting fluorescent signal probes. Notably, the utilization of Cas12a cis-cleavage activity, rather than trans-cleavage, eliminates the necessity for additional fluorescent probes, thus reducing interference between substances and enhancing sensitivity. Throughout the process, complementary DNA (cDNA) plays a crucial dual role in target recognition conversion and signal presentation, representing a key challenge and innovative aspect of this study. To evaluate the performance of the aptasensor, lysozyme (LYS) is employed as a representative model target of food allergens. Under optimal conditions, the developed aptasensor could achieve an exceptional low limit of detection (LOD) of 6.10 pM with a dynamic detection range of 10 pM-320 pM. The aptasensor demonstrates high selectivity and great recovery rates. This strategy yields promising outcomes, holding the potential to serve as a valuable reference for various food allergens detection.
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| http://dx.doi.org/10.1016/j.ijbiomac.2024.133444 | DOI Listing |
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