AI Article Synopsis
- Duplication plays a crucial role in molecular evolution and various diseases; researchers have developed a tool called Amplification Editing (AE) for precise DNA duplication.
- AE can duplicate substantial portions of human genomes, ranging from 20 bp to 100 Mb, with varying efficiencies depending on the size of the duplication.
- This tool has the potential to create microduplications in disease-related areas within embryonic stem cells, paving the way for advancements in cellular and animal model research.
Article Abstract
Duplication is a foundation of molecular evolution and a driver of genomic and complex diseases. Here, we develop a genome editing tool named Amplification Editing (AE) that enables programmable DNA duplication with precision at chromosomal scale. AE can duplicate human genomes ranging from 20 bp to 100 Mb, a size comparable to human chromosomes. AE exhibits activity across various cell types, encompassing diploid, haploid, and primary cells. AE exhibited up to 73.0% efficiency for 1 Mb and 3.4% for 100 Mb duplications, respectively. Whole-genome sequencing and deep sequencing of the junctions of edited sequences confirm the precision of duplication. AE can create chromosomal microduplications within disease-relevant regions in embryonic stem cells, indicating its potential for generating cellular and animal models. AE is a precise and efficient tool for chromosomal engineering and DNA duplication, broadening the landscape of precision genome editing from an individual genetic locus to the chromosomal scale.
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| http://dx.doi.org/10.1016/j.cell.2024.05.056 | DOI Listing |
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