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Floxing and Clinical Psychopharmacology.
J Clin Psychopharmacol
June 2024
From the Center for the Study of Drug Development, Tufts University School of Medicine, Boston, MA.
Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation.
Genome Biol
August 2019
Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia.
Background: CRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up to 16% efficiency in generating conditional knockout (cKO or floxed) alleles by microinjection of 2 single guide RNAs (sgRNA) and 2 single-stranded oligonucleotides as donors (referred herein as "two-donor floxing" method).
Results: We re-evaluate the two-donor method from a consortium of 20 laboratories across the world.
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