The content of chemical constituents in leaf extracts correlates positively with biological activities. The experimental objective was to carry out the phytochemical screening and purification of the major polyphenols from the leaves of . In addition, the anti- activity of the hydroalcoholic extract, fraction, subfractions and polyphenols purified were evaluated. After partitioning of the extract with ethyl acetate, the fractions were chromatographed on Sephadex LH-20 gel followed by RP-flash chromatography and monitored by TLC and RP-HPLC. The samples were characterized by mass spectrometry (LC-ESI-QTOF-MS) and subjected to the microdilution method in 96-well plates against strains of , and . Myricitrin (93.89%; /; / 463.0876), gallic acid (99.9%; /; / 169.0142), and ellagic acid (94.2%; /; / 300.9988) were recovered. The polyphenolic fraction (62.67% (/) myricitrin) and the ellagic fraction (67.86% (/) ellagic acid) showed the best antifungal performance (MIC between 62.50 and 500 μg/mL), suggesting an association between the majority constituents and the antifungal response of derivatives. However, there is a clear dependence on the presence of the complex chemical mixture. In conclusion, chromatographic strategies were effectively employed to recover the major polyphenols from the leaves of the species.
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http://dx.doi.org/10.3390/molecules29122761 | DOI Listing |
Cureus
December 2024
Department of Biochemistry, Era's Lucknow Medical College and Hospital, Era University, Lucknow, IND.
Background: Curcumin (Cur) is a polyphenol phyto-compound found in turmeric () that inhibits tumorigenesis by introducing apoptosis and restricting cell survival and proliferation. This in vitro research article focuses on the pharmacodynamic interactions of Cur combined with the commercial drug doxorubicin (Doxo) to enhance the cytotoxicity of Doxo at lower doses against triple-negative breast cancer cells (MDA-MB-231) with the chemo-protective effect against normal HEK293 cells. In this study, we observed the dose-dependent cytotoxicity, increased reactive oxygen species (ROS) generation, and increased chromatin condensation in combination doses compared to single doses.
View Article and Find Full Text PDFClin Pharmacol Ther
January 2025
Sydney Pharmacy School, Faculty of Medicine and Health, University of Sydney, Sydney, New South Wales, Australia.
Green tea (Camellia sinensis) is a commonly consumed beverage or dietary supplement. As a natural product with a myriad of proposed health benefits, patients are likely to consume green tea while taking their medications unaware of its potential to interact with drugs and influence drug efficacy and safety. Catechins are the abundant polyphenolic compounds in green tea (e.
View Article and Find Full Text PDFCompr Rev Food Sci Food Saf
January 2025
Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, China.
Many proteins are essential food components but also major allergens. Reducing protein allergenicity while preserving its nutritional value and technofunctional properties has always been the goal of the food industry. Ultrasound (US) is a green processing method for modifying proteins.
View Article and Find Full Text PDFFood Funct
January 2025
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
Whey protein (WP) contains two major allergenic proteins, α-lactalbumin and β-lactoglobulin, which significantly impact its incorporation and application in food products. Current research primarily focuses on the dynamic changes in allergenicity during the processing of individual protein components. To simulate realistic conditions in food processing, this study aims to investigate the effect of polyphenol oxidase cross-linking on the allergenicity of complex protein matrices.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Horticultural Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran.
In this study, screening of the collected 70 Salvia nemorosa L. populations from 54 habitats from West Azerbaijan province, Iran was evaluated by analyzing the content of phytochemical compounds, antioxidant activity, and UHPLC-HRMS profiling in different populations. The aerial parts of the plants were analyzed based on total phenolic (TPC) and flavonoid (TFC), total tannin (TTC), ascorbic acid (AAC), chlorophylls (Cla, and Clb), total carotenoid (TCC), β-carotene, antioxidant activity (by DPPH and FRAP assays), and 40 polyphenolic compounds by UHPLC-HRMS (phenolic acids, flavonoids and fatty acyl glicosides).
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