LM-1 Affects Multiple Cell Biological Processes in to Suppress Rice Blast.

, one of the most destructive rice pathogens, causes significant losses during the rice harvest every year. has been explored in many crops as a potential biocontrol agent. However, the mechanisms of controled rice blast are not fully understood. Here, a biocontrol strain LM-1, isolated from a contaminated medium, was identified as using morphological observation, physiological and biochemical tests, and 16S rDNA sequencing. LM-1 inhibited the growth and pathogenicity of and (Breda de Haan) Shoem. The mycelia of co-cultured with LM-1 were enlarged and broken by fluorescence microscopy using calcofluor white. LM-1 inhibited the mycelia of from producing conidia. Genes , , and were detected in LM-1. Furthermore, the supernatant of LM-1 interfered with the appressorium formation of , blocked conidial cell death, and reduced autophagy degradation but did not affect the normal germination of rice seeds and seeding growth. Additionally, we observed hypersensitivity reactions, reactive oxygen species, and iron accumulation reduction in rice cells inoculated with supernatant. Our study reveals that LM-1 has a control effect on rice blast and affects cell wall integrity, sporulation, appressorium formation, cell death, and autophagy.