AI Article Synopsis
- This study aimed to find effective protectants that enhance the survival of specific lactic acid bacteria (LAB) strains during spray drying, particularly focusing on enzymatic activity.
- It was discovered that the live cell counts of LAB were well-preserved in a 10% recovered skim milk group after spray drying, with pyruvate kinase (PK) and lactate dehydrogenase (LDH) proving more sensitive to the process than other enzymes.
- The research also resulted in four composite protectants that, when tested, showed that the combination of sodium glutamate, sucrose, and trehalose best preserved the viability and enzymatic activity of the LAB strain NCFM.
Article Abstract
The aim of this study was to identify the most effective protectants for enhancing the viability of specific lactic acid bacteria (LAB) strains ( subsp. CICC 6097, CICC 21839, NCFM) by assessing their enzymatic activity when exposed to spray drying (inlet/outlet temperature: 135 °C/90 °C). Firstly, it was found that the live cell counts of the selected LAB cells from the 10% (/) recovered skim milk (RSM) group remained above 10 CFU/g after spray drying. Among all the three groups (1% / RSM group, 10% / RSM group, and control group), the two enzymes pyruvate kinase (PK) and lactate dehydrogenase (LDH) were more sensitive to spray drying than hexokinase (HK) and β-galactosidase (β-GAL). Next, transcriptome data of NCFM showed that 10% (/) RSM improved the down-regulated expressions of genes encoding PK () and LDH () after spray drying compared to 1% (/) RSM. Finally, four composite protectants were created, each consisting of 10% (/) RSM plus a different additive-sodium glutamate (CP-A group), sucrose (CP-B group), trehalose (CP-C group), or a combination of sodium glutamate, sucrose, and trehalose (CP-D group)-to encapsulate NCFM. It was observed that the viable counts of strain NCFM (8.56 log CFU/g) and enzymatic activity of PK and LDH in the CP-D group were best preserved compared to the other three groups. Therefore, our study suggested that measuring the LDH and PK activity could be used as a promising tool to screen the effective spray-dried protective agent for LAB cells.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11205755 | PMC |
| http://dx.doi.org/10.3390/microorganisms12061094 | DOI Listing |
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