Actinoplanes kirromycinicus sp. nov., isolated from soil.

A novel actinomycete, designated as TPMA0078, was isolated from a soil sample collected in Shinjuku, Tokyo, Japan. 16S rRNA gene sequence analysis indicated that strain TPMA0078 belongs to the genus Actinoplanes and is closely related to Actinoplanes regularis IFO 12514 (99.86% 16S rRNA gene sequence similarity). The spores of strain TPMA0078 were motile, and the sporangia were cylindrical. The diamino acids in the cell wall peptidoglycan of strain TPMA0078 were meso-diaminopimelic acid and 3OH-meso-diaminopimelic acid. Whole-cell sugars were glucose and mannose, with galactose as a minor component. The major cellular fatty acids identified were iso-C, iso-C, and anteiso-C. The predominant menaquinone was MK-9(H), and the principal polar lipid was phosphatidylethanolamine. These chemotaxonomic properties of strain TPMA0078 were consistent with those of Actinoplanes. Meanwhile, digital DNA-DNA hybridization and average nucleotide identity values showed low relatedness between strain TPMA0078 and A. regularis NBRC 12514. Furthermore, several phenotypic properties of strain TPMA0078 distinguished it from those of closely related species. Based on its genotypic and phenotypic characteristics, strain TPMA0078 represents a novel species of the genus Actinoplanes, for which the name Actinoplanes kirromycinicus sp. nov. is proposed. The type strain is TPMA0078 (=NBRC 116422 = TBRC 18262).