AI Article Synopsis
- This study investigates the genes involved in the early developmental stages of the East Asian common octopus (Octopus sinensis) through RNA sequencing.
- Researchers identified 5,468 differentially expressed genes (DEGs) between hatched paralarvae and those 25 days post-hatching, revealing important factors affecting growth and development.
- The findings highlight key genes linked to processes like transmembrane transport and lipid biosynthesis, offering insights for improving octopus production and understanding their survival mechanisms.
Article Abstract
Background: The genes involved in cephalopod development and their association with hatching and survival during early life stages have been extensively studied. However, few studies have investigated the paralarvae transcriptome of the East Asian common octopus (Octopus sinen sis).
Objective: This study aimed to identify the genes related to embryonic development and hatching in O. sinensis using RNA sequencing (RNA-seq) and verify the genes most relevant to different embryonic stages.
Methods: RNA samples from hatched and 25 days post-hatching (dph) O. sinensis paralarvae were used to construct cDNA libraries. Clean reads from individual samples were aligned to the reference O. sinensis database to identify the differentially expressed genes (DEGs) between the 0- and 25-dph paralarvae libraries. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to supplement the RNA-seq data for embryogenic developmental stages.
Results: A total of 12,597 transcripts were annotated and 5,468 DEGs were identified between the 0- and 25-dph O. sinensis paralarvae, including 2,715 upregulated and 2,753 downregulated transcripts in the 25-dph paralarvae. Several key DEGs were related to transmembrane transport, lipid biosynthesis, monooxygenase activity, lipid transport, neuropeptide signaling, transcription regulation, and protein-cysteine S-palmitoyltransferase activity during the post-hatching development of O. sinensis paralarvae. RT-qPCR analysis further revealed that SLC5A3A, ABCC12, and NPC1 transcripts in 20 and/or 30 days post-fertilization (dpf) embryos were significantly higher (p < 0.05) than those in 10-dpf embryos.
Conclusion: Transcriptome profiles provide molecular targets to understand the embryonic development, hatching, and survival of O. sinensis paralarvae, and enhance octopus production.
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| http://dx.doi.org/10.1007/s13258-024-01537-3 | DOI Listing |
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Transcriptome analysis of East Asian common octopus, Octopus sinensis, paralarvae.
Genes Genomics
August 2024
Department of Marine Bioscience, Gangneung-Wonju National University, Gangneung, Gangwon-Do, 25457, Republic of Korea.
Article Synopsis
- This study investigates the genes involved in the early developmental stages of the East Asian common octopus (Octopus sinensis) through RNA sequencing.
- Researchers identified 5,468 differentially expressed genes (DEGs) between hatched paralarvae and those 25 days post-hatching, revealing important factors affecting growth and development.
- The findings highlight key genes linked to processes like transmembrane transport and lipid biosynthesis, offering insights for improving octopus production and understanding their survival mechanisms.
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