gen. nov., sp. nov. isolated from the world's deepest silver-uranium mine currently devoted to the extraction of radon-saturated water.

A Gram-stain-positive, rod-shaped, aerobic, motile bacterium, J379, was isolated from radioactive water spring C1, located in a former silver-uranium mine in the Czech Republic. This slow-growing strain exhibited optimal growth at 24-28 °C on solid media with <1 % salt concentration and alkaline pH 8-10. The only respiratory quinone found in strain J379 was MK-7(H). C ω9 (60.9 %), C (9.4 %), C and alcohol-C (both 6.2 %) were found to be the major fatty acids. The peptidoglycan contained directly cross-linked -diaminopimelic acid. Phylogenetic reconstruction based on the 16S rRNA gene sequences and the core-genome analysis revealed that strain J379 forms a separate phylogenetic lineage within the recently amended order . A comparison of the 16S rRNA gene sequences between strain J379 and other members of the order showed <96 % similarity. This analysis revealed that the closest type strains were D16/0 /H6 (95.2 %), 0166_1 (94.9 %) and KV-962 (94.5 %). Whole-genome analysis showed that the closest type strain was BR7-21 with an average nucleotide identity of 78 %, average amino acid identity of 63.2 % and percentage of conserved proteins of 48.2 %. The G+C content of the J379 genomic DNA was 71.7 mol%. Based on the phylogenetic and phylogenomic data, as well as its physiological characteristics, strain J379 is proposed to represent a type strain (DSM 113746=CCM 9300) of gen. nov. sp. nov. within the family .