AI Article Synopsis

  • Computer-aided design and manufacturing (CAD/CAM) techniques in dentistry use either subtractive or additive methods, focusing on the biocompatibility of methacrylate (MA)-based samples.
  • The study compared the effects of MA-CAD/CAM milled samples and 3D-printed samples on human gingival fibroblast viability and behavior over 2 and 24 hours.
  • Results showed that while CAD/CAM milled samples were biocompatible over 24 hours, 3D-printed samples led to significant decreases in cell viability, particularly after longer exposure times.

Article Abstract

Computer-aided design and computer-aided manufacturing (CAD/CAM) techniques are based on either subtractive (milling prefabricated blocks) or additive (3D printing) methods, and both are used for obtaining dentistry materials. Our in vitro study aimed to investigate the behavior of human gingival fibroblasts exposed to methacrylate (MA)-based CAD/CAM milled samples in comparison with that of MA-based 3D-printed samples to better elucidate the mechanisms of cell adaptability and survival. The proliferation of human gingival fibroblasts was measured after 2 and 24 h of incubation in the presence of these samples using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the membrane integrity was assessed through the lactate dehydrogenase release. The level of reactive oxygen species, expression of autophagy-related protein LC3B-I, and detection of GSH and caspase 3/7 were evaluated by fluorescence staining. The MMP-2 levels were measured using a Milliplex MAP kit. The incubation with MA-based 3D-printed samples significantly reduced the viability, by 16% and 28% from control after 2 and 24 h, respectively. There was a 25% and 55% decrease in the GSH level from control after 24 h of incubation with the CAD/CAM milled and 3D-printed samples, respectively. In addition, higher levels of LC3B-I and MMP-2 were obtained after 24 h of incubation with the MA-based 3D samples compared to the CAD/CAM milled ones. Therefore, our results outline that the MA-CAD/CAM milled samples displayed good biocompatibility during 24-h exposure, while MA-3D resins are proper for short-term utilization (less than 24 h).

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11204847PMC
http://dx.doi.org/10.3390/jfb15060147DOI Listing

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