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Transcriptome Analysis Reveals the Venom Genes of the Ectoparasitoid (Hymenoptera: Braconidae). | LitMetric

Transcriptome Analysis Reveals the Venom Genes of the Ectoparasitoid (Hymenoptera: Braconidae).

Insects

Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China.

Published: June 2024

AI Article Synopsis

  • * A study identified 34 venom proteins, including common types like serine protease and unique components such as a paralytic protein, emphasizing their roles in the parasitization process.
  • * The research confirmed high expression levels of these proteins in the venom apparatus, particularly highlighting the significance of the paralytic protein for effective host manipulation and potential applications in pest management.

Article Abstract

The ectoparasitoid (Hymenoptera: Braconidae) exhibits a broad parasitic capability towards various lepidopteran pests, with venom serving as a crucial virulent factor ensuring successful parasitization and subsequent host mortality. Analyzing the constituents of its venom is essential for elucidating the mechanisms underlying efficient host killing by this parasitoid and for exploring potentially functional venom proteins. Through a transcriptomic analysis, a total of 34 venom proteins were identified within the venom of , encompassing known components such as serine protease, metalloproteinase, esterase, and serine protease inhibitors commonly present in parasitoid venoms. Unique components like paralytic protein and ion transport peptide-like were identified, possibly specific to certain parasitoids, along with novel proteins with uncharacterized functions. Spatial gene expression profiling of the identified venom proteins using transcriptomic data, corroborated by quantitative PCR validation for 13 randomly selected proteins, revealed abundant expression levels in the venom apparatus, affirming them as genuine venom components. Notably, the paralytic protein exhibited prominent expression, with the highest FPKM (fragments per kilobase of transcript per million fragments mapped) value of 24,704.87 in the venom apparatus, indicative of its significant role in successful parasitism by . The identification of these venom proteins establishes a foundation for the further exploration of bioactive agents for pest management strategies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11203415PMC
http://dx.doi.org/10.3390/insects15060426DOI Listing

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