The first registered Itoh hybrid cv. Hexie in China is a naturally occurring intersectional hybrid of Sect. and Sect. . In this study, we sequenced, assembled, and analyzed the complete chloroplast genome of Itoh hybrid cv. Hexie. The result showed that the chloroplast genome of Hexie, with a typical circular tetrad structure, is 152,958 bp in length, comprising a large single copy (LSC) region of 84,613 bp, a small single copy (SSC) region of 17,051 bp, and two reverse complementary sequences (IRs) of 25,647 bp. The chloroplast genome encoded 116 genes, including 80 protein-coding genes, 32 tRNA genes, and 4 rRNA genes. Phylogenetic analysis inferred from the shared protein-coding genes showed that the Itoh hybrid cv. Hexie had the closest phylogenetic relationship with , followed by , indicating that was its maternal parent. This study provides a molecular resource for phylogenetic and maternal parent studies of Itoh hybrid, contributing to a basis for Itoh hybrid breeding strategies in the future.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11198146PMC
http://dx.doi.org/10.1080/23802359.2024.2368214DOI Listing

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