A method is presented for chemiluminescence resonance energy transfer (CRET) using APTES-FeO as a highly efficient energy acceptor with strong magnetic effectiveness over extended distances, while an Au@BSA-luminol composite acts as the donor. In order to boost the chemiluminescence reactions, CuO nanoparticles were successfully employed. The distance between the donor and acceptor is a crucial factor in the occurrence of the CRET phenomenon. A sensitive and high-throughput sandwich chemiluminescence immunosensor has been developed accordingly with a linear range of 1.0 × 10 g/L to 6.0 × 10 g/L and a limit of detection of 0.8 × 10 g/L. The CRET-based sandwich immunosensor has the potential to be implemented to early cancer diagnosis because of its high sensitivity in detecting Nanog, fast analysis (30 min), and simplicity. Furthermore, this approach has the potential to be adapted for the recognition of other antigen-antibody immune complexes by utilizing the corresponding antigens and their selective antibodies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00604-024-06505-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
CRET-based immunoassay on magnetic beads for selective and sensitive detection of Nanog antigen as a key cancer stem cell marker.
Mikrochim Acta
June 2024
Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, 1439817435, Iran.
A method is presented for chemiluminescence resonance energy transfer (CRET) using APTES-FeO as a highly efficient energy acceptor with strong magnetic effectiveness over extended distances, while an Au@BSA-luminol composite acts as the donor. In order to boost the chemiluminescence reactions, CuO nanoparticles were successfully employed. The distance between the donor and acceptor is a crucial factor in the occurrence of the CRET phenomenon.
View Article and Find Full Text PDFHighly chemiluminescent TiO/tetra(4-carboxyphenyl)porphyrin/N-(4-aminobutyl)-N-ethylisoluminol nanoluminophores for detection of heart disease biomarker copeptin based on chemiluminescence resonance energy transfer.
Anal Bioanal Chem
July 2019
CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Chemistry, University of Science and Technology of China, Hefei, 230026, Anhui, China.
In this work, the chemiluminescence (CL) property of 5,10,15,20-tetrakis(4-carboxyphenyl)-porphyrin- and N-(4-aminobutyl)-N-ethylisoluminol-functionalized TiO nanoparticles (TiO-TCPP-ABEI nanoluminophores) was studied for the first time. It was found that TiO-TCPP-ABEI nanoluminophores exhibited excellent CL activity in the presence of HO. The CL mechanism has been proposed due to the reaction of ABEI with HO and catalytic effect of TiO and TCPP.
View Article and Find Full Text PDFChemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine.
ACS Appl Mater Interfaces
July 2016
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.
We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid.
View Article and Find Full Text PDFAmorphous carbon nanoparticle used as novel resonance energy transfer acceptor for chemiluminescent immunoassay of transferrin.
Anal Chim Acta
March 2014
Key Laboratory of Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address:
Amorphous carbon nanoparticles (ACNPs) showing highly efficient quenching of chemiluminescence (CL) were prepared from candle soot with a very simple protocol. The prepared ACNP was employed as the novel energy acceptor for a chemiluminescence resonance energy transfer (CRET)-based immunoassay. In this work, ACNP was linked with transferrin (TRF), and horseradish peroxidase (HRP) was conjugated to TRF antibody (HRP-anti-TRF).
View Article and Find Full Text PDFIntroducing chemiluminescence resonance energy transfer into immunoassay in a microfluidic format for an improved assay sensitivity.
Chem Commun (Camb)
January 2012
Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China.
We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with a limit of detection at 3.6 × 10(-11) M E2 in a microfluidic format.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!