Genetic engineering technologies are essential not only for basic science but also for generating animal models for therapeutic applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system, derived from adapted prokaryotic immune responses, has led to unprecedented advancements in the field of genome editing because of its ability to precisely target and edit genes in a guide RNA-dependent manner. The discovery of various types of CRISPR-Cas systems, such as CRISPR-associated transposons (CASTs), has resulted in the development of novel genome editing tools. Recently, research has expanded to systems associated with obligate mobile element guided activity (OMEGA) RNAs, including ancestral CRISPR-Cas and eukaryotic Fanzor systems, which are expected to complement the conventional CRISPR-Cas systems. In this review, we briefly introduce the features of various CRISPR-Cas systems and their application in diverse animal models.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11278801 | PMC |
| http://dx.doi.org/10.1016/j.mocell.2024.100086 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Taming the wild: domesticating untapped northern fruit tree and shrub resources in the era of high-throughput technologies.
AoB Plants
January 2025
Department of Plant Science, McGill University, Macdonald Campus, 21111 Rue Lakeshore, Ste-Anne-de-Bellevue, H9X 3V9, Québec, Canada.
New crop`s need to emerge to provide sustainable solutions to climate change and increasing abiotic and biotic constraints on agriculture. A large breadth of northern fruit trees and shrubs exhibit a high potential for domestication; however, obstacles to implementing traditional breeding methods have hampered or dissuaded efforts for improvement. This review article proposes a unique roadmap for domestication of northern fruit crops, with a focus on biotechnological (e.
View Article and Find Full Text PDFAdvancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening.
Nat Commun
January 2025
Olivia Newton-John Cancer Research Institute, Heidelberg, Melbourne, Australia.
Cas12a is a next-generation gene editing tool that enables multiplexed gene targeting. Here, we present a mouse model that constitutively expresses enhanced Acidaminococcus sp. Cas12a (enAsCas12a) linked to an mCherry fluorescent reporter.
View Article and Find Full Text PDFEfficient secretory expression, purification, and characterization of lipase in Pseudomonas aeruginosa M18, with multifunctional applications in diagnostics.
World J Microbiol Biotechnol
January 2025
Zybio Inc, Chongqing, 400082, China.
Lipase (EC 3.1.1.
View Article and Find Full Text PDFsgRNA Single-Nucleotide Resolution by Ion-Pairing Reversed-Phase Chromatography.
Anal Chem
January 2025
Synthetic Molecule Design and Development, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, Indiana 46285, United States.
Single-stranded guide RNAs (sgRNAs) are important therapeutic modalities that facilitate selective genome editing by the CRISPR/Cas9 system. While these therapeutic modalities are synthesized through solid phase oligonucleotide synthesis similar to small interfering RNA (siRNAs) and antisense oligonucleotide (ASOs) therapeutics, their sequence length and complex secondary and tertiary structure hinder analytical characterization. The resulting current sgRNA methodologies have limited chromatographic selectivity near the FLP and limited MS compatibility.
View Article and Find Full Text PDFExosome-mediated CRISPR/Cas delivery: A cutting-edge frontier in cancer gene therapy.
Gene
January 2025
Cancer and Stem Cell Biology Laboratory, Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, TamilNadu 603203, India. Electronic address:
Cancer is considered the second most common disease globally. In the past few decades, many approaches have been proposed for cancer treatment. One among those is targeted therapy using CRISPR-Cas system which plays an irreplaceable role in translational research through gene editing.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!