AI Article Synopsis
- Synthetic hexaploid wheats (SHWs) are valuable for transferring important genes from wild relatives to common wheat.
- A chromosome-scale assembly for the SHW-derived cultivar 'Chuanmai 104' was created using advanced sequencing technologies, resulting in a genome size of 14.81 Gb and a high completeness score.
- This pioneering assembly provides a foundational resource to enhance wheat improvement efforts, addressing the growing global food demand.
Article Abstract
Synthetic hexaploid wheats (SHWs) are effective genetic resources for transferring agronomically important genes from wild relatives to common wheat (Triticum aestivum L.). Dozens of reference-quality pseudomolecule assemblies of hexaploid wheat have been generated, but none is reported for SHW-derived cultivars. Here, we generated a chromosome-scale assembly for the SHW-derived cultivar 'Chuanmai 104' based on PacBio HiFi reads and chromosome conformation capture sequencing. The total assembly size was 14.81 Gb with a contig N50 length of 58.25 Mb. A BUSCO analysis yielded a completeness score of 99.30%. In total, repetitive elements comprised 81.36% of the genome and 122,554 high-confidence protein-coding gene models were predicted. In summary, the first chromosome-level assembly for a SHW-derived cultivar presents a promising outlook for the study and utilization of SHWs in wheat improvement, which is essential to meet the global food demand.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11193762 | PMC |
| http://dx.doi.org/10.1038/s41597-024-03527-2 | DOI Listing |
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