Direct observation by the naked eye of fluorescence-stained microbes adsorbed on surface imprinted polymers (SIPs) is highly challenging and limited by speed, accuracy and the semiquantitative nature of the method. In this study, we tested for the presence of spores of f. sp. race 4 (4), which cause severe banana Fusarium wilt disease and reduces the area of banana plants. This kind of spore can become dormant in soil, which means that the detection of secreted molecules (molecular imprinting) in soil may be inaccurate; detection methods such as polymerase chain reaction (PCR) and Raman spectroscopy are more accurate but time-consuming and inconvenient. Therefore, a semiquantitative and rapid SIP detection method for 4 was proposed. Based on the ITO conductive layer, a reusable and naked-eye-detectable 4-PDMS SIP film was prepared with a site density of approximately 9000 mm. Adsorption experiments showed that when the 4 spore concentration was between 10 to 10 CFU/mL, the number of 4 spores adsorbed and the fluorescence intensity were strongly correlated with the concentration and could be fully distinguished by the naked eye after fluorescence staining. Adsorption tests on other microbes showed that the SIP film completely recognized only the series. All the results were highly consistent with the naked-eye observations after fluorescence staining, and the results of the 4-infected soil experiment were also close to the ideal situation. Taken together, these results showed that 4-PDMS SIPs have the ability to rapidly and semiquantitatively detect the concentration of in soil, which can provide good support for banana cultivation. This method also has potential applications in the detection of other fungal diseases.
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http://dx.doi.org/10.1021/acsami.4c06275 | DOI Listing |
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